Selection of the composition of the nutrient medium and cultivation conditions of Saccharomyces cerevisiae, a producer of feed protein

The aim of the research was to study the selection of the composition of the nutrient medium and the parameters of cultivation of the protein producer - yeast Saccharomyces cerevisiae VKPM Y-365. The possibility of growth of a microbial protein producer - Saccharomyces cerevisiae VKPM Y-365 on a modified nutrient medium (wort replaced with sugar solution, that is, fermentolysate of cereal straw) in the volume necessary to obtain 10 g/l of glucose is shown. The growth rate of the microbial producer on the nutrient medium recommended by the passport of the microorganism, containing instead of wort fermentolysate of cereal straw, on the 6th day of cultivation is 17-19% higher than on the nutrient medium with wort. During periodic cultivation in deep conditions on the recommended and modified nutrient medium of the producer of the Saccharomyces cerevisiae feed protein VKPM Y-365, the consumption of easily fermentable sugars by a microorganism in an environment with buckwheat straw fermentolysate was 96%, which is 16.4% more than in the recommended medium; yeast cell growth of 9.8 McF - 40% more; yeast yield, 44% of the initial glucose concentration is 46.6% higher; the yield of "raw" protein 47% in a.c.c is 42.4% higher, respectively. The optimal conditions for the cultivation of Saccharomyces cerevisiae, a producer of feed protein, are the glucose concentration in the nutrient medium of 43.35 g / l, the sowing dose of the microbial producer is 1.5 McF, and the duration of cultivation is 125 hours. At the same time, the concentration of yeast biomass was 5.81 g/l. The steep ascent can be considered effective, since the result obtained is 10% higher than the theoretically calculated one. In the feed biomass obtained by cultivating a microbial producer on a modified nutrient medium, the content of B vitamins and vitamin D3 is on average 45-75% higher than when grown on a control nutrient medium. The obtained data formed the basis of the technology for producing feed protein, where a sugar solution based on cellulose-containing waste serves as a component of the nutrient medium for the microbial producer of feed protein - yeast strain Saccharomyces cerevisiae VKPM Y-365.