Search of the genes responsible for biosynthesis of guanidine toxins in bacteria of Bacillus sp 1839 and Pseudoalteromonas sp 2138

In the current research the bioinformatic analysis of 32 open reading frames (ORF) in the plasmid pNe-1, involved in TTX synthesis in bacteria Aeromonas sp. isolated from puffer fish, was performed. The search of genes from pNe-1 in plasmid DNA of TTX-producing strains Bacillus sp. 1839, Pseudoalteromonas sp. 2138 and a total bacterial DNA from puffer fish Takifugu sp. was also carried out. ORFs with the high level of conservation were identified. These ORFs encode amino acid sequences identified to «hypothetical proteins», which can be involved in TTX and its analogs synthesis. The obtained results can be recommended for genetic studies of the guanidine toxins biosynthesis.