Obtaining rabies virus antigen and assessment of its activity and specificity in immunosorbent assay and immunoblot

This article contains the rationale for the preparation and purification of the rabies virus antigen by the homogenization on FastPrep-24, followed by a conversion in a sucrose density gradient. It has been established that the maximum purified antigenic fractions are located in the 1520% sucrose zone. As a result, a major fraction of polypeptides was isolated, a high antigenic activity of which was confirmed by the methods of ELISA and immunoblot. In prospect it can be used as an immunizing material and as a component of rapid test-systems for the diagnosis of rabies.