DH-plant production in culture of unpollinated ovules of cucumber (Cucumis sativus L.)

Relevance. The development of F1 hybrids distinguishing it from cultivars by high productivity, plant uniformity in ripening date, fruit sizes and quality is the promising trend in breeding program in cucumber (Cucumis sativus L.). The aim of the study was to optimize the gynogenesis induction condition in culture of unpollinated ovules in vitro in order to broad the generation of new breeding forms and to accelerate homozygous line production. Materials and methods. Eight promising cucumber accessions from Laboratory of Cucurbit Breeding and Seed Production (FSBSI FSVC) were taken for the study. The protocol developed in Laboratory of Biotechnology (FSBSI FSVC) for production of doubled haploid in Cucurbitaceae family was used in the experiment. The medium IMC with 30 g/L sucrose and 7g/L agar supplemented with 200 mg/L ampicillin and 0.2 mg/L thidiazuron (TDZ) was applied to induce gyno-genic development. Results. The half-open bud or flower was shown to be the most suitable to be taken as an explant for cultivation...