Obtaining and purifying anticharbonneux immunoglobulines

The aim of the research was to obtain active and specific immunoglobulines and fluorescent serum for indication and identification of anthrax in biological objects. In hyperimmunization, the five - fold scheme of protective anthrax antigen injection was used. Interval between the injections was 7 days. Immunoglobulin fracture from hyperimmune serum was allocated through ammonium sulphate and the chromatography with DEAE cellulose. Control of activity was performed by indirect ELISA. This method allowed us to eliminate the main quantity of ballast proteins and to get a high-specific globulin for diagnostic systems.