Obtaining recombinant nucleocapsid protein of PPR virus for disease serodiagnostic

Peste des petits ruminants is a highly contagious, acute or subacute viral disease of sheep and goats, characterized by fever, sores in the mouth, haemorrhagic gastroenteritis, lesions in lymphatic system and pneumonia. (E.P.J. Gibbs et al., 1979; A. Diallo et al., 1989; T.M. Ismail et al., 1995). Because of high morbidity of 50-100 % and mortality of 50-90 %, Peste des petits ruminants belongs to a number of emerging diseases, having a significant threat to livestock production in countries where the disease is notified (R.A. Kock et al., 2015; E.M.E. Abu-Elzein et al., 1990). The etiological agent of PPR is a Morbillivirus (PPRV) of Paramyxoviridae family (M.H.V. Van Regenmortel et al., 2000). The PPRV antigens are similar to antigens of other Morbilliviruses (G. Libeau et al., 2014). Severity of the clinical signs depends on different factors, e.g. PPRV line, animal species, breed, and immune status. Because of that, the final diagnosis must be confirmed by laboratory methods...