Liquid fermentation of Stagonospora cirsii C-163, a potential mycoherbicide for Cirsium arvense (L.) Scop

The bioherbicides should exhibit stable effectiveness in the field, be specific and quick in action, compatible with other preparations and meet market demand. In many ways, the cost and quality of product is determined by the technology of obtaining infectious material. An infectious material is used as a mycelium and its modifications and as conidia as well. The extreme sensitive of the mycelium to drying is often referred as main disadvantage of using it as the basis of a formulation. At the same time, the technological process of obtaining conidia is often more complicated, and the efficiency in the field is less than the mycelium has. The phytopathogenic fungus Stagonospora cirsii J.J. Davis, which is causative agent of a leaf spot disease of creeping perennial weeds in the family Asteraceae, is considered a potential mycoherbicide of Canadian thistle Cirsium arvense (L.) Scop. However, the yield of C. cirsii C-163 mycelium on standard nutrient media is significantly lower than that used in biotechnology (3 g/l)...