Obtaining hydrosoluble peptides from the biomass of Musca domestica larvas and study of their properties

The aim of the research was the isolation of hydrosoluble peptides from the biomass of M. domestica larvae and the study of their antimicrobial activity. To achieve this goal, the following tasks were set: to select high performance liquid chromatography (HPLC) conditions for effective separation of hydrosolublepeptides, to isolate peptides from the biomass of M. domestica larvae, to study antibacterial activity. Thehydrosoluble peptides with 0.1 M phosphate-buffered saline were extracted according to original technique developed by the authors, consisting of successive stages, such as resolution, centrifugation, and multiple salting out of proteins with ammonium sulfate. Later the conditions for the separation of hydrosolublepeptides by HPLCon a BioSep SEC S-2000 300x2120 mm column at a flow rate of 1 ml / min and a wavelength of 280 nm were selected, chromatography time of 60 minutes, the volume of the injected sample was 20 μl and the temperature of 25 ºC. Thus, 6 protein fractions from the biomass of Muscadomestica larvaewe were obtained...