Reproduction of Russian sturgeon (Acipenser gueldenstaedtii) viable juveniles using cryopreserved sperm and behavioral reactions of the cryo-progeny

Cryopreservation of male reproductive cells is an important issue of genetic biodiversity conservation strategy and the development of fisheries and aquaculture. The use of cryopreserved semen in artificial reproduction and aquaculture will provide genetically diverse progeny, reduce area and the cost of maintaining male fishes, and, thereby, will allow rise in female herd abundance. Cryopreserved sperm can be used at any time, without risk of untimely maturing or improper quality. The data on the low-temperature preservation indicates that the long-term storage in liquid nitrogen does not significantly affect the safety of cells after freezing and thawing. The viability of cryopreserved sperm is usually confirmed by laboratory fertilization of caviar, without further observations of the obtained juveniles. This paper reports for the first time the behavioral response of pre-larvae, larvae, and juveniles of Russian sturgeon ( Acipenser gueldenstaedtii von Brandt & Ratzeburg 1833), obtained with the use of frozen-thawed sperm, and shows physiological fullness of the resulting progeny...