Polymorphism of pea storage protein convicilin
Автор: Selikhova T.N., Bobkov S.V.
Журнал: Вестник аграрной науки @vestnikogau
Статья в выпуске: 4 (49), 2014 года.
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Pea convicilin could hinder physico-chemical properties of protein isolates. Pea accessions without convicilin or with its unusual isoforms could be used in pea breeding on high quality of storage proteins. Polymorphism of convicilin was studied with use of 60 accessions of genus Pisum L. Standard SDS-PAGE electrophoresis was used for separation of storage proteins. In total, four isoforms of convicilin were revealed. They were located on positions 14, 15, 16 and 17 of electrophoretic spectra. Isoform 15 was found with high frequency (0.35). The frequency of isoform 17 was equal to 0.32. Differences between cultivated and wild pea accessions on frequency of convicilin isoforms were determined. Among 6 breeding lines and varieties of Р. sativum only two (14 and 17) isoforms were revealed. Isoform 17 was represented with the highest frequency (0.83). In wild subspecies of Р. sativum (24 accessions) only three (15, 16, 17) isoforms were found. Isoform 15 had the highest frequency (0.6). In accessions of wild species Р. fulvum all four revealed isoforms of convicilin were represented. Isoform 15 was found preferably (0.59). In variety Aest electrophoretic analysis revealed one seed which did not contained convicilin. This seed was characterized by distinguished from other seeds spectrum.
Pea, sds-page, storage protein, accession, pea breeding
Короткий адрес: https://sciup.org/147124167
IDR: 147124167
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