Polymorphism of pea storage protein convicilin

Автор: Selikhova T.N., Bobkov S.V.

Журнал: Вестник аграрной науки @vestnikogau

Статья в выпуске: 4 (49), 2014 года.

Бесплатный доступ

Pea convicilin could hinder physico-chemical properties of protein isolates. Pea accessions without convicilin or with its unusual isoforms could be used in pea breeding on high quality of storage proteins. Polymorphism of convicilin was studied with use of 60 accessions of genus Pisum L. Standard SDS-PAGE electrophoresis was used for separation of storage proteins. In total, four isoforms of convicilin were revealed. They were located on positions 14, 15, 16 and 17 of electrophoretic spectra. Isoform 15 was found with high frequency (0.35). The frequency of isoform 17 was equal to 0.32. Differences between cultivated and wild pea accessions on frequency of convicilin isoforms were determined. Among 6 breeding lines and varieties of Р. sativum only two (14 and 17) isoforms were revealed. Isoform 17 was represented with the highest frequency (0.83). In wild subspecies of Р. sativum (24 accessions) only three (15, 16, 17) isoforms were found. Isoform 15 had the highest frequency (0.6). In accessions of wild species Р. fulvum all four revealed isoforms of convicilin were represented. Isoform 15 was found preferably (0.59). In variety Aest electrophoretic analysis revealed one seed which did not contained convicilin. This seed was characterized by distinguished from other seeds spectrum.

Еще

Pea, sds-page, storage protein, accession, pea breeding

Короткий адрес: https://sciup.org/147124167

IDR: 147124167

Список литературы Polymorphism of pea storage protein convicilin

  • Bobkov S.V., Lazareva T.N. (2012): Chemical composition of electrophoretic spectra of seed storage proteins of interspecific hybrids of peas. Genetics, 48: 56-61.
  • Bobkov S.V., Lazareva T.N. (2012): Band composition of electrophoretic spectra of storage proteins in interspecific pea hybrids. Russian Journal of Genetics, 48: 47-52.
  • Bourgeois M., Jacquin F., Savois V., Sommerer N., Labas V., Henry C., Burstin J. (2009): Dissecting the proteome of pea mature seeds reveals the phenotypic plasticity of seed protein composition. Proteomics, 9: 254-271.
  • Byrne O.M., Hardie D.C., Khan T.N., Speijers J., Yan G. (2008): Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum × P. fulvum interspecific cross. Aust. J. Agric. Res., 59: 854-862.
  • Casey R., Domoney C. (1999) Pea globulins. In: Seed Proteins. In: Shewry R.P., Casey R. (Eds): Cluwer Academic Publishers, Amsterdam, the Netherlands, 171-208.
  • Ellis T.H.N., Domoney C., Castelon J., Cleary W., Davis D. (1986): Vicilin genes of Pisum. Mol. Gen. Genet., 205: 164-169.
  • Hildebrand C.E., Torney D.C., Wagner R.P. (1992): Informativeness of polymorphic DNA markers. Los Alamos Science, 20: 100-102.
  • Identification of varieties and registration of the genofond of cultivated plants by seed proteins (2000): In: KONAREV V.G. (ed.): VIR, St. Petersburg. (in Russian)
  • Jha S.S., Ohri D. (2002): Comparative study of seed protein profiles in the genus Pisum. Biol. Plant., 45: 529-532.
  • Loridon K., Mcphee K., Morin J., Dubreuil P., Pilet-Nayel M.L., Aubert G., Rameau C., Baranger A., Coyne C., Lejeune-Henaut I., Burstin J. (2005): Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theoretical and Applied Genetics, 111: 1022-1031.
  • Makasheva R.Kh. (1979): Grain Legume Crops. In: Korovina O.N. (ed.): Kul'turnaya flora SSSR (The Flora of Cultivated Plants of the Soviet Union), Kolos, Leningrad, 1979, 44-50. (in Russian)
  • Nisar M., Ghafoor A., Khan M.R., Asmatullah (2009): First proteomic assay of pakistani Pisum sativum L. Germplasm relation to geographic pattern. Russ. J. Genet., 45: 805-810.
  • O’kane F.E., Happe R.P., Vereijken J.N., Gruppen H., Van Boekel M.A.J.S. (2004): Characterization of pea vicilin: 1. denoting convicilin as the α-subunit of the Pisum vicilin family. Agricultural and Food Chemistry, 52: 3141-3148.
  • Ochatt S.J., Benabdelmouna A., Marget P., Aubert G., Moussy F., Pontécaille C., Jacas L. (2004): Overcoming hybridization barriers between pea and some of its wild relatives. Euphytica, 137: 353-359.
  • Tzitzikas E.N., Vincken J.P., Groot J., Gruppen H., Vissar R.G.F. (2006): Genetic variation in pea seed composition. J. Agric. Food Chem., 54: 425-433.
Еще
Статья научная