Повышение производительности цикла проектирование-создание-тестирование-обучение систем (DBTL) в синтетической биологии растений (обзор)

Повышение производительности цикла проектирование-создание-тестирование-обучение систем (DBTL) в синтетической биологии растений (обзор)

Автор: Prasad S.S., Das U.

Журнал: Сельскохозяйственная биология @agrobiology

Рубрика: Обзоры, проблемы

Статья в выпуске: 5 т.59, 2024 года.

Бесплатный доступ

Синтетическая биология растений - это молодая научная дисциплина, которая объединяет принципы инженерии с биологией с целью разработки уникальных систем на основе растений для различных применений, от производства биотоплива до улучшения сельскохозяйственных культур. Эта технология может революционно изменить традиционное сельское хозяйство, содействовать устойчивому развитию и решению глобальных проблем продовольственой безопасности, изменения климата и возобновляемых источников энергии. Цикл проектирование-создание-тестирование-обучение (Design-Build-Test-Learn, DBTL) обеспечивает основу для процесса планирования, разработки, оценки и совершенствования синтетических биологических систем. Он позволяет исследователям итеративно настраивать производительность биологических цепей, что делает этот цикл критически важным инструментом для построения сложных биологических систем с предсказуемым и надежным поведением. Однако на каждом этапе этого цикла могут проявиться узкие места из-за неэффективного проектирования, ограниченного запаса генетических компонентов, технических проблем при разработке и управлении биологическими системами и трудностей в корректном мониторинге производительности системы. Для преодоления узких мест в цикле DBTL можно использовать различные стратегии: совершенствование вычислительных технологий для эффективного проектирования, расширение набора генетических компонентов, повышение точности и масштабируемости приемов редактирования генома и внедрение методов высокопроизводительного скрининга для точного измерения производительности системы. В этой обзорной статье мы обсудим последние достижения в улучшении производительности цикла DBTL для преодоления его узких мест.

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Цикл dbtl, мультиомика, биоортагональные реакции, микрофлюидика, изотопные трассеры, loica, crispr

Короткий адрес: https://sciup.org/142243772

IDR: 142243772   |   DOI: 10.15389/agrobiology.2024.5.831rus

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