Practical importance of polymerase chain reaction for the detection and genotipiving Pasteurella multocida

The results of the study of practical significance of the method of identifying and determining the genotype of pathogenic variants of Pasteurella multocida bacteria were presented. It included carrying out polymerase chain reaction (PCR) with subsequent electrophoretic detection, transfer of amplification products to the gel and analysis of the reaction. The study of samples was carried out in two reactions using selected pairs of oligonucleotide primers. The first made it possible to detect theP. multocida bacterium and determine the serogroups A and D, and the second B, Ε and F. The sensitivity of the method was 103 CFU / ml and 105 CFU / g of tissue, respectively. This method identified and typed the bacterium P. multocida of five capsular groups - A, B, D, E and F. One can examine pure and mixed cultures, as well as samples of biological material from animals. The bacteria genome was detected in 126 samples, which accounted for 48.5 % of the total number of studied biomaterial samples...