Предварительный анализ гемоцианинов в гемолимфовой плазме эндемичных амфипод Байкала
Автор: Бедулина Д.С., Гурков А.Н., Бадуев Б.К., Борвинская Е.В., Димова М.Д., Тимофеев М.А.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 1 т.12, 2016 года.
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Двумерный электрофорез белков плазмы гемолимфы нескольких эндемичных видов байкальского амфипод показал, что гемоцианин является основным основным белком в плазме этих видов. Изоформы гемоцианинов образуют на гелях максимум 24 отдельных пятна с несколько иной молекулярной массой и меняются по их изоэлектрическим точкам. Анализ транскрипционных последовательностей гемоцианина байкальского амфиполя Eulimnogammarus verrucosus выявил широкую гетерогенность изоформ гемоцианина, которые были разделены на две различные группы по их аминокислотной последовательности и имеют разную расчетную молекулярную массу и изоэлектрические точки. Бета-тип гемоцианина впервые описан для перкаридных ракообразных.
Короткий адрес: https://sciup.org/14323987
IDR: 14323987
Список литературы Предварительный анализ гемоцианинов в гемолимфовой плазме эндемичных амфипод Байкала
- Bajkalovedenie (Baicalogy) (2012) Nauka, Novosibirsk. 1114 p.
- Bradford M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-254
- Camacho C., Coulouris G., Avagyan V., Ma N., Papadopoulos J., Bealer K. & Madden T.L. (2009) BLAST+: architecture and applications. BMC Bioinformatics, 10(1), 421
- Decker H. and Foll R. (2000) Temperature adaptation influences the aggregation state of hemocyanin from Astacus leptodactylus. Comp Biochem Physiol A Mol Integr Physiol., 127(2), 147-54
- Decker H. and van Holde K. (2011) Oxygen and the evolution of life. Springer-Verlag Berlin Heidelberg. 172 p
- Decker H., Hellmann N., Jaenicke E., Lieb B., Meissner U. and Markl J. (2007) Recent progress in hemocyanin research. Integrative and Comparative Biology, 47, 631-644
- deFur P., Mangum C. and Reese J. (1990) Respiratory responses of the blue crab Callinectes sapidus to long-term hypoxia. Biological Bulletin, 178, 46-54
- Hagner-Holler S., Kusche K., Hembach A. and Burmester T. (2005) Biochemical and molecular characterisation of hemocyanin from the amphipod Gammarus roeseli: complex pattern of hemocyanin subunit evolution in Crustacea. Journal of Comparative Physiology B, 175, 445-452
- Hampton S.E., Izmest'eva L.R., Moore M.V., Katz S.L., Dennis B. & Silow E.A. (2008) Sixty years of environmental change in the world's largest freshwater lake-Lake Baikal, Siberia. Global Change Biology, 14(8), 1947-1958
- Huang X. and Madan A. (1999) CAP3: A DNA sequence assembly program. Genome Res., 9, 868-877
- Kozlowski L.P. (2007-2016) Isoelectric Point Calculator. http://isoelectric.ovh.org
- Kravtsova L.S., Izhboldina L.A., Khanaev I.V., Pomazkina G.V., Rodionova E.V., Domysheva V.M., Sakirko M.V., Tomberg I.V., Kostornova T.Ya., Kravchenko O.S. & Kupchinsky A.B. (2014). Nearshore benthic blooms of filamentous green algae in Lake Baikal. Journal of Great Lakes Research, 40(2), 441-448
- Laemmli U.K. (1970) Most commonly used discontinuous buffer system for SDS electrophoresis. Nature, 227, 680-685
- Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., Valentin F., Wallace I.M., Wilm A., Lopez R., Thompson J.D., Gibson T.J. and Higgins D.G. (2007) ClustalW and ClustalX version 2. Bioinformatics, 23(21), 2947-2948
- Mangum C.P. (1997) Adaptation of the oxygen transport system to hypoxia in the blue crab, Callinectes sapidus. American Zoologist, 37, 604-611
- Mangum C.P., Greaves J. and Rainer J.S. (1991) Oligomer composition and oxygen binding of the hemocyanin of the blue crab Callinectes sapidus. Biological Bulletin, 181, 453-458
- Marchler-Bauer A., Lu S., Anderson J. B., Chitsaz F., Derbyshire M. K., DeWeese-Scott C.,... & Gwadz M. (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic acids research, 39 (suppl 1), D225-D229
- Moore M.V., Hampton S.E., Izmest’eva L.R., Silow E.A., Peshkova E.V. & Pavlov B.K. (2009) Climate Change and the World’s ‘Sacred Sea’ -Lake Baikal, Siberia. BioScience, 59(5), 405-417
- O'Farrell P.H. (1975) High resolution two-dimensional electrophoresis of proteins. Journal of biological chemistry, 250(10), 4007-4021
- Petersen T.N., Brunak S., von Heijne G. & Nielsen H. (2011) SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature Methods, 8, 785-786
- Scherbaum S., Ertas B., Gebauer W. and Burmester T. (2010) Characterization of hemocyanin from the peacock mantis shrimp Odontodactylus scyllarus (Malacostraca: Hoplocarida). Journal of Comparative Physiology B, 180, 1235-1245
- Schneider C.A., Rasband W.S., Eliceiri K.W. (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods, 9, 671-675
- Spicer J. and Hodgson E. (2003) Structural basis for salinity-induced alteration in oxygen binding by haemocyanin from the estuarine amphipod Chaetogammarus marinus (L). Journal of the Marine Biological Association of the UK, 83, 945-947
- Takhteev V.V., Berezina N.A., Sidorov D.A. (2015) Checklist of the Amphipoda (Crustacea) from continental waters of Russia, with data on alien species. Arthropoda Selecta, 24(3), 335-370
- Tamura K., Stecher G., Peterson G., Filipski A., Kumar S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729
- Terwilliger N.B. and Ryan M. (2006) Functional and phylogenetic analyses of phenoloxidases from brachyuran (Cancer magister) and branchiopod (Artemia franciscana, Triops longicaudatus) crustaceans. Biological Bulletin, 210, 38-50
- Terwilliger, N.B. (2015) Oxygen transport proteins in Crustacea: hemocyanin and hemoglobin. Physiology, 4, Oxford University Press. 512 p
- Timofeyev M.A. (2010) Ecological and Physiological Aspects of Adaptation to Abiotic Environmental Conditions of Endemic Baikalian and Palearctic Amphipods. Thesis for Dr.Sci. degree. Tomsk, 384.
- van de Braak C.B.T., Faber R. & Boon J.H. (1996) Cellular and humoral characteristics of Penaeus monodon (Fabricius, 1798) haemolymph. Comparative haematology international, 6(4), 194-203
- van Holde K.E. and Miller K.I. (1995) Hemocyanins. Advances in Protein Chemistry, 47, 1-81
- Vaquer-Sunyer R. and Duarte C. (2008) Thresholds of hypoxia for marine biodiversity. Proceedings of the National Academy of Sciences of the USA, 105, 15452-15457
- Voit R., Feldmaier-Fuchs G., Schweikardt T., Decker H. and Burmester T. (2000) Complete sequence of the 24mer hemocyanin of the tarantula Eurypelma californicum: structure and intramolecular evolution of the subunits. The Journal of Biological Chemistry, 275, 39338-39344