Preparative isolation of specific antigens of full cell lysate M. bovis

Bovine tuberculosis is a serious problem for agriculture all over the world and causes huge economic losses. At the same time, infections caused by Mycobacterium bovis not only affect animal husbandry and the food industry, but also pose a threat to public health, as M. bovis is often isolated as a pathogen of tuberculosis, especially in patients with various immune deficiencies. The situation is aggravated by the lack of simple and accessible methods for diagnosing tuberculosis in cattle, which significantly impedes the monitoring of infection and the application of appropriate preventive measures. Serological tests, due to their relatively cheap cost, rapidity, rather high sensitivity and specificity, are able to compete with the allergic test during mass examinations of cows. In the present study, we present the results on the study of the antigenic structure of mycobacteria and the isolation of the most specific antigenic components from the lysate of the M. Bovis cell wall destroyed on the Fast Prep 24 device. The developed method allows obtaining a serologically active antigenic fraction with a high degree of purity with a molecular weight of 28 kDa.