Use of aminodiphosphonates for prophylaxis of calcification of epoxy-treated bioprostheses

Studied were the prospects of aminodiphosphonate (AOPDPA) in reducing calcium-linking activity of epoxy-treated cardiovascular biological prostheses. Bovine pericardium, porcine aortic valve leaflets and walls of aorta preserved with gluteraldehyde (GA) and ethylene glycol diglycidyl ether (EGDE) were used in the experiment. Calcium-linking activity, structure and physical and mechanical properties of the biomaterial were estimated. The use of AOPDPA to modify bovine pericardium and aortic valve leaflets preserved with EGDE results in significant reduction of calcium-linking activity without any effect on the structure and mechanical properties of the biomaterial. The efficacy of anticalcification protection with AOPDPA immobilized on the aortic wall tends to decrease with time and is ified in the end. Exclusion of the aortic wall from bioprosthetic structure may be considered a way to prevent calcification of heart valve bioprostheses.