Application of high-resolution melting analysis and MALDI-minisequencing for polymorphism genotyping of human paraoxonase-1 gene

High-resolution melting analysis (HRMA) and MALDI minisequencing were used to perform single nucleotide polymorphism (rs854560 and rs662) genotyping of human paraoxonase-1 gene. The allelic frequencies (66.0 % for L-allele and 71.8 % for Q-allele) obtained from genotyping of 94 habitants of Kirov region were similar to allelic frequencies of Europeans and Caucasian Americans. The experimental data confirmed the applicability of HRM analysis and MALDI minisequencing for genotyping of human paraoxonase-1 L55M and Q192R single nucleotide polymorphisms.