Usage of molecular markers for identification of CMS-Rf system in parental lines of sunflower hybrids

The testing results of molecular markers of CMS-Rf system in samples from the sunflower lines collection of the All-Russia Research institute of oil crops are presented. The purpose of the work was to determine a set of DNA-markers for identification of nucleus gen-restorer of pollen fertility Rf1 and mitochondrial gen of cytoplasmic male sterility orfH522 in lines and inbreds. There were used 16 inbred lines of the different usage direction: maternal, paternal and maintainers of sterility. Six pairs of primers were tested, they were created for amplification of marker fragments of DNA for gens Rf1 and orfH522. A set from three pair of primers for identification of gens Rf1 and orfH522 was determined as a result of the conducted work. Its use in breeding and seed growing practices is recommended.