The trace elements various sources use in diets in combination with and without phytase and their effect on zootechnical indicators and intestinal microbiocenosis of broiler chickens

The purpose of research is to study the effect of organic forms of microelements in combination with and without phytase on the productive indicators of broiler chickens, intestinal microbiocenosis and its histoarchitectonics. As a result of an experiment conducted in 2021 in the vivarium of the Federal Scientific Center VNITIP of the Russian Academy of Sciences on 4 groups of broiler chickens of the Ross-308 cross, it was found that the use of organic forms of microelements in compound feeds in the form of L-asparaginates in an amount of 7.5 % of guaranteed norms contributed to better assimilation of metals, this had a positive effect on the physiological, productive and production indicators of poultry. In broilers, the diet of which contained a complex of organic forms of microelements in combination with phytase, the productivity indicators were higher in comparison with chickens of other groups, which was a confirmation of the better assimilation of feed nutrients by the bird under the influence of the enzyme introduced into the diet. In the study of the intestinal microbiome of broiler chickens that consumed feed with the addition of organic forms of trace elements in the form of L-asparaginates, both with and without phytase, already at the level of phyla, shifts were noted both in the composition of the microbiome and in the level of its biodiversity, which is also indirectly could affect the productive performance of poultry. Cluster analysis made it possible to establish a great similarity of the microbiome of the caecum of the intestines in poultry of groups III and IV, the diet of which included 7.5 % of trace elements in the form of L-asparaginates from the accepted norms, both with the addition of phytase (group III) and without it (group IV)). Histological studies have shown that the organic form of trace elements (OMEK-broiler) in the form of L-asparaginates did not significantly affect the change in the histoarchitectonics of the intestine, and the addition of phytase against the background of asparaginates contributed to the smoothing of mild mononuclear infiltration.