Protective Effects of Wheat Grass on Histopathology of Some Organs and Biomarkers Parameters Against Lead Acetate Toxicity in Wistar Rats

Protective Effects of Wheat Grass on Histopathology of Some Organs and Biomarkers Parameters Against Lead Acetate Toxicity in Wistar Rats

Автор: Mansouri Ouarda, Zohra Hamamdia, Cherif Abdennour

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 3 т.17, 2021 года.

Бесплатный доступ

This study focuses on the search for a natural treatment to reduce lead toxicity in male Wistar rat by using the aquous extract of wheatgrass Triticum aestivum (WG). In addition to the control group (C), rats were exposed to a diet containing 600 mg Pb acetate/Kg diet (Pb), or combined with the WG at 9g/100g diet (Pb-WG) for a period of 6 weeks. Biochemical, reproductive and histological markers were evaluated. The results of haematological parameters show a very significant increase in white blood cells and lymphocytes with the group treated with the lead single. By contrast there was no difference was recorded between the treated group by Pb-WG and the control. A significant decrease in the red blood cells, the haemoglobin and haematocrit was recorded in the group treated with Pb alone. The tests of hormonal and biochemical parameters showed a decrease in the concentration of the hormone triiodothyronin (T3) and thyreostimulin (TSH) in the Pb group compared to the (control and Pb-WG). The serum testosterone concentration, urea, total cholesterol levels, the rate of TGP, TGO and creatinine were significantly increased in rats treated with (Pb) alone compared to (the control and Pb-bl group), while no change in glucose was shown. However, the (Pb-WG) group shows no change compared to the control. The level of calcium showed a significant decrease in the Pb group, and which returns to the normal state in group (Pb-WG) compared to the control. These results are confirmed by the study of histological sections. A morphological change represented by volume shrinkage of the vesicles. A return to the normal structure of follicles was observed in (Pb-WG) group. Histology study of the kidney, testis epididymis showed no change in (Pb-WG) compared to the control group. Contairement in the group exposed to lead (Pb) the parenchyma of the kidney shows dilated distal and proximal tubules causing renal tubular damage. The testes marked destruction and degeneration of germ cells and the light of some seminiferous tubules are empty. The study of the fertility parameters indicates a highly significant decrease in the concentration, the mobility of sperm counts among the treated group by Pb alone. The administration of the wheat grass has increased.

Еще

Hematological, biochemical markers, fertility, TSH, Testosterone and histological sections, Pb, wheatgrass (WG)

Короткий адрес: https://sciup.org/143173905

IDR: 143173905

Список литературы Protective Effects of Wheat Grass on Histopathology of Some Organs and Biomarkers Parameters Against Lead Acetate Toxicity in Wistar Rats

  • Adeniyi T.T, Ajayi G.O, Akinloye O.A. (2008). Effect of Ascorbic acid and Allium sativum on tissue lead in female Rattus navigicus, J. Health Biomed. Sci. 7(2): 38-41.
  • Adraoui I., Aziz A. (2007). Détermination des métaux lourds par voie électrochimique. J. les technologies de laboratoire. 3: 16.
  • Aleksandra K., Sławomir K., Stanisław H., Alina O., Ewa G.M, Ewa R., Anita O. et Ewa B. (2007). Assessment of semen function and lipid peroxidation among lead exposed men, Toxicology and Applied Pharmacology. 228(3): 378-384.
  • Amdur M.O, Doull J., Klaassen C.D. (1996). Lead.In Casarrett and Doull’s Toxicology, 5eéd. New York. McGraw-hill.
  • Anjum M.R., Madhu P., Reddy K.P., Reddy P.S., (2017). The protective effects of zinc in leadinduced testicular and epididymal toxicity in Wistar rats. Toxicology and Industrial Health, 33 (3), 265-276.
  • Ann Wigmore, (2001). L'herbe de blé : Source de santé et de vitalité, Editions Jouvence: 160.
  • Apostoli P., Kiss P., Porru S.J.P. et Vanhourne M. (1998). Occup Environ, Med. 55: 364-374.
  • Ardelt B., et al., (2001). Chlorophyllin protects cells from the cytostatic and cytotoxic effects of quinacrine mustard but not of nitrogen mustard, Int.J.Oncol. 18: 849-53.
  • Ashry K.M, El-Sayed Y.S, Khamiss R.M, El-Ashmawy I.M. (2010). Oxidative stress and immunotoxic effects of and their amelioration with myrrh (Commiphoramolmol) emulsion, Food ChemToxicol. 48(1): 236–241.
  • ATSDR (Agency for Toxic Substances and Disease Registry), (1999). Toxicological profile for lead, US Department of Health and Human Services: 640.
  • Bellinger D.C. (2008). Very low lead exposures and children’s neurodevelopment current opinion in Pediat. 20: 172-177.
  • Bonnard N., Falcy M., Hesbert A., Jargot D., Pillière F., Schneider O., Serre P. (2006). Plomb et composés minéraux Fiche établie par les services techniques et médicaux de l’INRS, FT. 59: 7-8.
  • Botta A., Poyen D., Signouret M. et Mathias A. (1976). Les différents tests biologiques de dépistage d'une imprégnation saturnine applicables en médecine du travail, Arch. Mal Prof. 37: 4-5, 437-443.
  • Boloor K.K et al., (2000). Chlorophyllin as a protector of mitochondrial membranes against gamma-radiation and photosensitization, J.Toxicology. 155: 63-71.
  • Bowers W. F. (1947). Chlorophyll in Wound Healing and Suppurative Disease, Am. J. Surg. 73: 37–50.
  • Bunn T.L, Ladics G.S, Holsapple M.P. (2001). Developmental immunotoxicology assessment in the rat.Age.Gender and strain comparisons after exposure to Pb, Toxicol.Met. 11: 41-58.
  • Cobot S. (2006). The liver tests, http://www./Weighcontrodoctor.Com/ healthtopics/healthyliverbowelbook: 98.
  • Dieter, D. and Joann, E. (1998):"Textbook of Veterinary Histology. "5th ed., Waverlg Company, Paris, Hong Kong,Sydney, Tokyo.
  • Ehle A.L, McKee D.C. (1990). Neuropsychological effect of lead in occupational exposed workers, Crit. Rev. Toxico. 20: 237-255.
  • El-Sayed Y.S., El-Neweshy M.S. (2009). Impact of lead toxicity on male rat reproduction at “hormonal and histopathological levels”, Toxicol Lett. 189 (Suppl.1): 219–20.
  • Evans T.J., (2015). Reproductive toxicity and endocrine disruption of potential chemical warfare agents. Handbook of Toxicology of Chemical Warfare Agents, Elsevier. pp. 599-613.
  • Fasce C.F. (1982). Serum Cholesterol determined colorimetrically with enzyme. Clin. Chem, 18: 901
  • Finkelstein Y., Markowitz M.E., and Rosen J.F. (1998). Low-level lead-induced neurotoxicity in children: an update on central nervous system effects, Brain Res Rev. 27(2): 168-176.
  • Flora S., Flora G. et Saxena G. (2006). Environmental occurrence, health effects and management of lead poisoning, In: J.S. Casas and J. Sordo, Editors lead chemistry, analytical aspects, environmental impact and health effects, Elsevier Science, Amsterdam, Netherlands: 158–228.
  • Gardan M.J, Lausanne E. (2010). Comment renforcer les défenses immunitaires par la nutrithérapie ? 2: 6-74.
  • Gargouri M., Soussi A., Akrouti A., Magné C., El Feki A., (2019). Potential protective effects of the edible alga Arthrospira platensis against lead-induced oxidative stress, anemia, kidney injury, and histopathological changes in adult rats. Appl. Physiol. Nutr. Metab. 44, 271-281.
  • Garnier R. (2011). Toxicité du plomb et de ses dérivés inorganiques, Diplôme Interuniversitaire (DIU) de Toxicologie Médicale.
  • Georing PL. (1993). Lead- protein interaction as a basis for toxicity, Neurotoxicol. 14: 45-60.
  • Goldstein GW. (1993). Evidence that lead acts as a calcium substitute in second messenger metabolism, Neurotoxicology. 14(3): 97-103.
  • Hanan Salah Eldeen Eldamaty (2020). Protective effects of barley and wheat grasses on nephrotoxicity in rats and some biochemical parameters induced by tramadol. Egypt. J. of Nutrition and Health Vol. 15 No. 1
  • Hala MFED, Mohamed SS, El-Messery TM (2014) Role of the functional food (pomegranate-yoghourt) as hepatoprotective effect on liver injured rats. Int J Curr Microbiol App Sci 3(8):185–196.
  • Heide L. (1991). Traditionelle Arzneipflanzen in der Gesundheitsversorgung der dritten Welt-Möglichkeiten und Grenzen. Zeitschrift für Phytotherapie, 12: 1-8.
  • Hughes et Letner (1936). Chlorophyll and Hemoglobin Regeneration, American Journal of Medical Science: 188-206.
  • INSERM (1999). Plomb dans l’environnement. Quels risques pour la santé?, Expertise collective. Paris: 461.
  • IPCS (1995). Environmental health criteria 165, Inorganic lead. Geneva. WHO: 300.
  • IFCC (International Federation of Clinical Chemistry), (1980). Recommandation of IFCC Methods of the measurement of catalytic concentrations of Enzymes, Clin. Chem. Acta, 105: 147-157.
  • Isherwood D. (1979). Med. Lab. Sci. 36: 211-235.
  • Jackie T., Haleagrahara N., Chakravarthi S., (2011). Antioxidant effects of Etlingeraelatior flower extract against lead acetate-induced perturbations in free radical scavenging enzymes and lipid peroxidation in rats. BMC Res. 4, 67-75.
  • Janmeda P., Sharma V., Singh L., Paliwal R., Sharma S., Yadav S. (2011). Chemopreventive Effect of Hydro-Ethanolic Extract of Euphorbia neriifolia Leaves against DENA-Induced Renal carcinogenesis in Mice, Asian Pacific J. Cancer Prev.12: 677-683
  • Jarrar B.M. (2003). Histological and histochemical alterations in the kidney induced, by Ann Saudi Med. 23 (1–2): 10–15.
  • Kumar S.S et al., (2001). Scavenging of reactive oxygen species by chloropyllin: an ESR study, Free Rad Res. 35: 563-74.
  • Lai C.N. (1979). Chlorophyll: The active factor in wheat sprout extract inhibiting the metabolic activation of carcinogens in vitro, Nutrition and Cancer.1 (3): 19-21.
  • Lakhanpal R., Davis J., Typpo J., Briggs G. (1966). Evidence for an unidentified growth factor from alfalfa and other plant sources for young guinea pigs, J. Nutr. 89: 341-346.
  • Lockitch G., (1993). Perspective on lead toxicity, Cline. Biochem. 26: 371-381.
  • Mabrouk A., (2018). Therapeutic effect of thymoquinone against lead-induced testicular histological damage in male Wistarrats, Andrologia. 50, 13014.
  • Manish V., Vasudha S., Somnath S. (2009). Evaluation of antioxidant profile and activity of amalaki (Emblicaofficinalis), spirulina and wheat grass, Indian Journal of Clinical Biochemistry. Feb. 24(1): 70-75.
  • Mansouri O., Abdennour C. (2011). Evaluation of the therapeutic efficiency of raw garlic on reproduction of domestic rabbits under lead induced toxicity, Scholars Research Library Annals of Biological Research. 2(3): 389-393.
  • Mansouri O., Abdennour C. (2008). Influence of Sudden Cystine Supplementation and Suppression on Adrenal and Ovary of Lead Exposed Rat. EuroJournals Publishing, Inc. 23(4): 548-558.
  • Marawaha R.K, Bansal D., Kaur S., Trehan A. (2004). Wheatgrass Juice Reduces Transfusion Requirement in Patients with Thalassemia Major: A Pilot Study, Indian Pediatric. 41: 716-720.
  • Marc S. and Béchir S. (2006). Guide technique de l’assainissement, 3é édition. le moniteur. Paris: 30.
  • Mary, Y. S. (2003). "Competinghigh cholesterol with hypothyrodism. "www about hypothyrodism.Com.
  • Mehta A., Flora S.J. (2001). Possible role of metal redistribution, hepatotoxicity and oxidative stress in chelating agents induced hepatic and renal metallothionein in rats, Food Chem. Toxicol. 39: 1029-1038.
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp