Gene construct-based serine protease of bacillus pumilus as a feed additive for poultry farming

Bacterial enzymes, in particular proteinases, as dietary additives in poultry farming, improve digestibility of feed nutrients and, as a consequence, make animal diets cheaper. This explains why bioadditives are being actively developed worldwide. Proteinаses break down proteins and reduce the negative effect of digestive inhibitors thus allow the costs for purchasing synthetic amino acids to be lower. Bacteria and microscopic fungi, including those with gene constructs developed to increase yield and improve properties of the expressed enzymes, may be producers. Bacterial serine proteinases have a high thermostability and are resistant to inhibitors of animal origin. In this paper, we report for the first time about the production of highly purified secreted subtilisin-like serine proteinase from Bacillus pumilus upon expression of the recombinant vector in B. subtilis strains and evaluate the main physicochemical and biological characteristics of the synthesized product...