Protistocidal activity of cationic surfactants and known antiprotozoal drugs

The purpose of the study is to assess the prospects of using fatty acid amides as antiprotozoal agents in comparison with known drugs. The paper presents the results of experimental research on the study of the protistocidal action of cationic surfactants synthesized in the laboratory by our creative team of amides of myristic and oleic acids and known in the world on the market of antiprotozoal drugs: trichopolum (metronidazole), toltrazuril, antimalarial drug chloroquine (delagil) and antibiotic bicillin 5. The protistocidal activity of substances was determined in a liquid nutrient medium by the method of serial dilutions according to our method, repeatedly presented in high-ranking literary sources. The test culture for the study was protozoans (ciliates) of the Colpoda steinii species. As a result of the study, it was noted that at a concentration of amidamiristic acid solution of 4.63 μg/ml and higher, the death of protozoa occurred almost immediately (within 1-2 minutes), the minimum protistocidal concentration oleic acid amide for colpods was 9.25 µg/ml at 2 min exposure; chloroquine protistocidal activity appeared after 1 h 30 min at a concentration of 15.63 µg/ml, and after 18 h exposure kolpods died at a concentration of 1.95 µg/ml. The minimum level of protistocidal activity of the reference drug toltrazuril is 22.5 μg/ml at an exposure of 19.5 hours, Trichopolum (metronidazole) and antibiotic bicillin 5 - 250 μg/ml at an exposure of 18 hours and 19 hours, respectively. These results show that myristic amide and oleic acid amide synthesized by us make it possible to use lower concentrations of solutions with a minimum exposure for parasitocidal action in comparison with known protistocidal preparations.