Expansion and identification of the potato cyst-forming nematode in agro-landscapes of the Republic of Karelia

The expansion of the quarantine object Globodera rostochiensis Woll., the disease producing factor of potato globoderesis was studied on the territory of 16 municipal districts of the Republic of Karelia in the period from 2012 to 2016. The selection of soil samples was carried out on the land plots belonging to different forms of property: agricultural enterprises, private and farm households. The nematode cysts were retrieved by the use of the funnel method. The viable cysts were used. The identification of G. rostochiensis was conducted with the help of morphometrical and molecular analysis methods. The research results showed that the soil share infected by globoderesis was not more than 46,7 % in agricultural enterprises and farm households. A part of the tested areas in Olonetsky, Prionezhsky, and Sortavalsky districts were free of phytohelminth. In Muyezersky, Segezhsky, Pudozhsky, Kondopozhsky, Pitkyarantsky, and Suoyarvsky districts the percent of such soils reached from 71% to 100%. Belonging of the cyst-forming nematode to the species of G. rostochiensis was identified on the basis of vulvar pallets analysis. The Granik index varies from 2,3 to 8,7 mkm. On the territory of the Republic of Karelia the expansion of the areal of Globodera rostochiensis Woll is conditioned by the use of susceptible breeds, the lack of nematocides, violation of quarantine measures, and ets.