Regenaration of Larix sibirica Ledeb. in in vitro culture through the somatic embryogenesis and the effect of mycorhyza on its growth in soil

The article presents the results of the analysis of Larix sibirica Ledeb. reproduction in in vitro culture through the somatic embryogenesis and the effect of mycorrhiza on their growth in the soil environment. To obtain microclonal Siberian larch it is effective to use 1 mg / L 2,4-D (2,4-dichlorophenoxyacetic acid) and 2 mg / L 6-BAP (6-benzylaminoperemedin) as growth regulators with pH-5.8. The dosage for preparation of mycorrhiza according to the manufacturer’s instructions was 3.25 ml with the addition of 15 ml of water. In microclonal plants that have been infected with mycorrhiza, lateral roots are formed from the main roots of the plants, and the average number of branched roots in 15 plants is 8.8 cm.The average length of the main root is 6.7 cm and 3.4 cm longer than in plants that have not been infected with mycorrhiza. The survival rate of microclonal plants is 64. Thus, mycorrhizae increase the amount of water and nutrients absorbed by the plant root and create a connection between roots and soil, branching the plant roots over a wider area.