The study of bacteria, isolated from the salt mining of Upper-Kama potassiummagnesium salt deposit

Using a method of enrichment, twelve strains of bacteria belonging to different taxonomic groups were isolated from soil, water and salt mine wastes from the salt mining area, of Solikmsk in the Perm region. The analysis of REP-PCR and ARDRA showed that isolated strains formed five genomic groups. Phylogenetic analysis based on 16S rDNA sequences indicated that isolates belonged to the genera Bacillus, Virgibacillus, Pseudomonas, Chromohalobacter and Idiomarina. Two strains, identified as belonging to the genera Bacillus and Streptomices, were isolated from a rock sample originating from a potassium-magnesium salt deposit. Microorganisms, closely related to the genus Ralstonia and Stenotrophomonas (98% identity) were found in the enrichment culture, obtained from the rock specimen with the use of medium supplemented with 20% NaCl. The majority of the strains grew at 15-20% NaCl in cultural medium, two strains, designated С2 and К52 grew at alkaline conditions (pH 9.0). Strain Pseudomonas sp. К513 used benzoic acid at high concentration (5 g/l) as a growth substrate at 3 % of NaCl.