DNA melting data processing techniques development

The conditions and parameters of the implementation of the DNA melting techniques were chosen. There were developed the DNA melting data processing techniques with advanced models based on sigmoidal nonlinear function and its derivative, a model based on a Gaussian function, a model based on power polynomials of degree 3 and 7. The comparison of the results of the application of techniques for processing real data of 8 samples received on the device ANK-32 was carried out. An algorithm for computing the full automation in MATLAB software environment was developed. The possibility of the separation of systematic and random error are shown. Developed techniques used to determine the temperature unevenness in nucleic acid analyzer tubes.