The development of real-time PCR method for indication and identification of Vibrio parahaemolyticus

Bacteria V. parahaemolyticus - gram-negative halophilic bacteria that can be the cause of acute intestinal disease in humans, occurring as food toxicoinfection. The success of the treatment-and-prophylactic and sanitary-anti-epidemic measures aimed at preventing of "halophiles" spreading largely depends on timely and accurate identification of Vibrio cholerae strains isolated from patients, seafood and under monitoring of environmental objects. This article presents the development of a method for identifying V. parahaemolyticus strains by Real-Time PCR, which includes the isolation of chromosomal DNA from the test material, setting up the PCR method with constructed primers specific to the site of the collagenase gene vppC and probe, allowing to detect the amplified fragment and to record the results in real time. The possibility of indication and identification of microorganisms of V. parahaemolyticus species under the study of samples of biological material (fish, seafood) based on the Real-Time PCR method is shown.