DEVELOPMENT OF A DEVICE FOR SETTING UP AND CHECKING OPTICAL SYSTEM OF GENETIC ANALYZER FOR MASSIVE PARALLEL SEQUENCING
Автор: E. K. Vasil’eva, I. V. Chubinskiy-Nadezhdin, V. E. Kurochkin, Y. I. Alekseev, Yu. V. Kulikov
Журнал: Научное приборостроение @nauchnoe-priborostroenie
Рубрика: Научные статьи, посвященные памяти Л.Н. Галль
Статья в выпуске: 4, 2024 года.
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The development of a device for adjusting and testing the optical system of a DNA sequencer for massively parallel sequencing (MPS) is described. The device contains a microfluidic cell with fluorescent microspheres immobilized on the flat surface of the chip channel. Microspheres with a diameter of 1 μm serve as a model of fluorescent colonies of DNA fragments formed during amplification and subsequent sequencing. A method for immobilizing a stable monolayer of microspheres on the surface of the chip channel is considered.
Sequencer DNA, DNA colonies, fluorescent microspheres, microfluidic cell, optical system, digital images
Короткий адрес: https://sciup.org/142242732
IDR: 142242732
