Peritoneal fluid cells reactivity in modeling of aseptic inflammation

The aim of investigation was to evaluate the characteristics of the response of rat peritoneal leukocytes and mast cells (MC) when using experimental aseptic inflammation. Material and Methods: Rats of the 1st group (n=7) were intra-peritoneally injected with 1 % thioglycolate broth, animals ofthe2nd(n=7)- the same broth, diluted 1:10. Control group rats (n=8) received an equal volume of saline. After removing animals from the experiment in the peritoneal fluid, the number of leukocytes was counted and their viability was evaluated. MC degranulation was detected by toluidine blue technique. All MCs were divided according to the degree of degranulation. Results: A model of aseptic inflammation of the peritoneum was reproduced. It was found that the number of leukocytes of peritoneal fluid, especially the number of non-viable cells, increases by more than 2 times with an increase in the concentration of the eluting agent (p