Redox status of peripheral blood erythrocytesin experimental ovarian cancer on the background of autohemochemotherapy with cisplatin and doxorubicin

Objective of the study was to evaluate the redox status of peripheral red blood cells in experimental ovarian cancer on the background of autohemochemotherapy (AHCT) with cisplatin and doxorubicin.Material and methods. In erythrocytes of 120 white outbred rats with transplantable ascitic ovarian cancer (AOC) the intensity of lipid peroxidation (LPO) was evaluated by the level of diene conjugates, ketodienes, Schiff bases and malondialdehyde at 3 and 8 days after intravenous infusion and AHCT with cisplatin and doxorubicin in doses 75 and 40 mg/m2, respectively. Results and discussion. Rats with AOC have an increase in the level of lipid peroxidation products while reducing the activity of catalase and glutathione transferase, which indicates the occurrence of oxidative stress. A significant increase in the amount of protein oxidative modification products thus indicates the development of carbonyl stress. The infusion of cisplatin and doxorubicin using AHCT significantly reduces the levels of lipid peroxidation products in the studied parameters in contrast to conventional intravenous infusion of chemodrugs, in which the change in the levels of lipid peroxidation products was multidirectional. The activity of catalase and SOD increased on the background of the use of both methods, and the activity of GT - only when using AHCT. These data suggests the elimination of oxidative stress effects in red blood cells of rats with AOC on the background of AHCT with cisplatin and doxorubicin. Effect of cisplatin and doxorubicin on the redox status of erythrocytes in experimental ovarian cancer in rats depends on the chemotherapy infusion route. In conventional intravenous infusion, the phenomenon of oxidative stress persists, but the carbonyl stress phenomenon is eliminated. The AHCT, in contrast, eliminates oxidative but retains carbonyl stress condition.