Regenerative ability of integumentary tissues when using fractions of waste products of G. mellonella larvae

The aim of the study is to determine the effect of integumentary tissue regeneration using fractions of the waste products of G. mellonella larvae under conditions of an experimental model of an incised wound. The studies were conducted at the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences and at the Department of Epizootology and Veterinary-Sanitary Expertise of Udmurt State Agrarian University in 2021-2022. The object of the study was aqueous solutions of fractions of the waste products of G. mellonella larvae. Laboratory mice were divided into pairs of analogs. The experiment modeling of an incised wound of the integumentary tissues of animals was carried out under anesthesia, followed by an incision with a scalpel and resection of a skin area according to a 15 mm template. The appearance of the wound was assessed daily, the change in the wound surface area was recorded (according to the method of J.I. Kundin), and the timing of wound healing was noted. The indicators were recorded on the 1st, 7th, 10th and 20th days. The phases of the wound process were determined by visual assessment of the defect on a three-point scale. Comparative assessment of the phases in the experimental groups was performed using photograph analysis and the method of linear assessment of the defect size. In the control, a tendency for a slight increase in CFU was noted throughout the experiment. In the experimental groups, when using aqueous extracts, the number of CFU on the 7th and 10th days was less than in the control, by 62 and 73.5 %, respectively. When sanitizing with a solution of the light fraction, CFU is 2.5 % higher than in the control. At the end of the experiment, on the 20th day, it was revealed that when using an aqueous solution of the light fraction of pancreas, the scar is thin, barely noticeable, the connective tissue is not differentiated, light pink in color. When treating the wound surface with an aqueous solution of the heavy fraction of pancreas, it was found that there is more connective tissue than in mice of the 1st experimental group. There is no hair on the wound surface, the wound surface is incompletely closed, and is replaced by connective tissue. Aqueous solutions have a positive effect on tissue regeneration. The difference in wound area values in the control with the 1st experimental group is 7 times, in the 2nd group - 1.4 times.