Retrospective analysis of 536 embryological protocols of cryocycles: the influence of oocyte competence and genetic screening of human embryos on vitrification results

The achievements of fundamental science have recently become the most widespread in the field of reproductive medicine. The biology of human development has mostly become understandable and manageable, thanks to new technologies. Some stages of the early human embryogenesis are predictably reproduced in the laboratories of assisted reproductive technologies (ART). Nevertheless, despite all the achievements, the success (birth of a healthy child) does not exceed 30% of the started cycles. That is why there is a continual search for new methods and their combinations to achieve better results and develop standard protocols for managing patients with infertility. The purpose of this work is to analyze the feasibility of genetic screening of embryos (NGS) and to compare the effectiveness of using donor and own oocytes when transferring a single embryo in cryocycles. We have analyzed the 536 cryocycles. There were four groups of patients with single embryo transfer (SET): group 1 - cycles with NGS, embryos with genetically euploid status taken for selection for transfer with used own oocytes (NSd, n=20); group 2 - cycles with single embryo transfer without NGS and using own oocytes (nSd, n=446); group 3 - cycles without NGS, using donor oocytes (nSD, n=8). All groups 1, 2, and 3 were near the same with an average age (34,1 - 34,3 - 34,6 years, respectively). Due to the small size of group 3, another (4th) observation group was taken (cycles without NGS, using donor oocytes, nSD, n=62), but without age restrictions, where the average age of patients was 42.3 years. Analyze of the survival rate shows significant decreasing in the group used donor cells (3 groups) than in groups (1 and 2) used own cells (84.62% vs 100%, p=0.060 and 91.96%, p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }