The role of hyperparathyroidism in the immune status forming at chronic renal failure (experimental study)

Objective: To study the role of hyperparathyroidism in the immune status forming of patients with chronic renal failure and the effects of parathyroid hormone on immune cells. Hypothesis testing was performed on 72 white non male rats divided into 4 groups: 1 - intact animals, 2 - sham-operated animals, 3 - animals with chronic renal failure (CRF), 4 - animals with experimental hyperparathyroidism. CRF in rats was modeled by a two-stage operative resection of 5/6 kidney tissue, hyperparathyroidism - by synthetic hyperphosphatic diets during 120 days. In peripheral blood we evaluated the quantitative composition of leukocytes, the functional activity of phagocytes by absorptive capacity and the generation of reactive oxygen in spontaneous and induced Nitro Blue Tetrazolium Reduction Test. Th1- and Th2-dependent response was evaluated according to a delayed type hypersensitivity reaction and the number of antibody-forming cells in the spleen after immunization. It was found that experimental chronic renal failure results in neutrophilia and lymphopenia in peripheral blood, activated absorption capacity and generation of reactive oxygen forms in peripheral blood phagocytes, depression of Th1- and Th2-dependent adaptive immune