Salicylic Acid and Salt Stress Tolerance in Plants: A Review
Автор: Reyaz Ahmad Mir, Aryendu R. Somasundaram
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 3 т.17, 2021 года.
Бесплатный доступ
Soil salinity has become a hot debate and has gained a great scientific interest towards global food security for an increasing population. Because salinity has numerous negative effects on crop physiology and results loss of productivity. Various attempts to overcome salinity have led to detrimental effects. However, scientists have been recruited to use certain eco-friendly techniques to increase stress tolerance in plants by rising the emission rate. One of them, which is naturally present in certain plant species, is Salicylic acid, a well-known growth regulator. Since plant hormones can monitor plant development and growth, they also serve as a strong protector against various abiotic stresses. But their concentration decreases beyond the level of protection when exposed to abiotic stressors such as NaCl stress. Thus, by increasing the amount of endogenous SA, externally supplied SA has found considerable beneficial effects on plant stress resistance. SA introduces many physiological, biochemical, and molecular modifications to plants under salt stress that include immunity. Evidence of external application of SA under salt stress tolerance in plants is discussed in this review. In addition, low SA concentrations have also been addressed and its cross-talk with other regulators.
Salicylic acid, Salt stress, Antioxidants, Hormonal cross-talk
Короткий адрес: https://sciup.org/143173901
IDR: 143173901
Текст научной статьи Salicylic Acid and Salt Stress Tolerance in Plants: A Review
The adverse impact on agricultural production, lead by environmental stress such as salinity has developed considerable scientific attention. The research community has made several attempts to counteract the harmful symptoms of abiotic stress in crop physiology. lants' adaptability and protection are hindered by abiotic stresses such as drought and salt stress, which induce physiological and cellular changes. Salt stress is a common and devastating abiotic stressor that affects plant growth success, limiting crop production around the world (Munns and Tester, 2008; Mimouni et al. , 2016; Qiu et al. , 2017; Debnath et al. , 2018). Currently, about 20% of the world's cultivated land is affected by salt, with 2 million ha (roughly 1%) estimated to be deteriorating each year due to salinity (Qiu et al. , 2017; Ke et al. , 2018; El-Ramady 11 et al. , 2019). FAO reported that 19% of the world's land suffered from salinity in excess of 6% (FAO and IT S, 2015). Salinity is projected to trigger losses of over 50 percent of agriculture by 2050. The human population is growing exponentially and requires a significant rise in worldwide agricultural production. roductivity must be improved by 70 percent with an additional 2.3 billion individuals by 2050 to fulfill the burden of feeding the target population (Tilman et al. , 2011). Therefore, efforts to improve salt resistance in crops are crucial to ensure targeted food protection globally.
The immediate result of NaCl stress is osmotic stress due to the presence of ions surrounding the roots, preventing the extraction of water by the roots and reducing the growth of the plant. However, secondary damage (lead by ion toxicity) can also occur as a result of redox state changes and oxidative burst by ROS species production (Mittal et al. , 2012; Mostofa et al. , 2015; Nahar et al. , 2016). ROS accumulation is commonly cited as a key eventual outcome of aerobic metabolism (Sharma et al. , 2012). Excessive generation after its detoxification capability, however, exposes the plant to oxidative stress, an extremely dangerous physiological disorder that can potentially contribute to lipid peroxidation, protein oxidation, nucleic acid damage, main metabolic enzymes inhibition, and programmed cell death (Sharma and Dubey, 2005;
Kamanga et al. , 2018).
A variety of plant physiological activities such as osmotic stress, ion disruption, nutritional disturbances, membrane damage, cell division and expansion reduction, and genotoxicity were disrupted by high salinity (Abogadallah, 2010; Safdar et al. , 2019). Together, these results minimize the growth of plants, and consequently the yield. However, plants use an inborn defensive mechanism to combat ion toxicity, such as the aggregation or exclusion of inorganic ions, the control of the absorption of ions by the roots and their transport through the leaves, the activation of antioxidant defense mechanisms linked to ROS, the accumulation of osmolytes and the modification of the biological membrane structure ( irasteh-Anosheh et al. , 2016). This effectiveness persists up to a definite degree above which plants felt and ultimately died of water shortage (secondary drought). In addition, upsurged salinity level is thought to typically cause decreased synthesis and degradation of plant growth regulators ( GRs) in most cases (Hayat and others 2010). This contributes to total growth decline and in some cases, plant death also.
For instance, exogenous applied SA that can enhance stress tolerance in plants under saline stress maybe some of these strategies. Numerous experiments have shown that SA has added altered concentrations of sodium and potassium to various plant species and increased grain yields. For example, maize (Gunes and others 2005), tomato (Szepesi 2006; He and Zhu 2008), wheat (Kaydan and others 2007), and barley ( irasteh-Anosheh and others 2015; akar and others 2016), respectively. When used at low doses, Arif, et al. , (2020) proposed in their literature (review) that SA may serve as a potent phytohormone that regulates several physiological and other aspects in plants.
hytohormones are naturally occurring organic compounds that have a profound impact on plant physiological processes at low doses. hyto-regulators play a significant role in fostering plant growth, both under normal conditions and under stress as well. Several experiments have shown that each plant hormone does not play a single biological function in plants, but plays a complex and successful role at various levels, in different tissues or under different environmental conditions (Ku, et al., 2018; Yang et al., 2019; Cortleven et al., 2019). In this review, summarised and illustrated the multifunctional role of salicylic acid in crops during salt stress have been discussed.
SA (a novel growth regulator) improves resistance to NaCl stress:
Salicylic acid (SA) is a phenolic compound derived from plants that serve as a growth regulator as well as a primary defense mechanism. It was discovered and isolated in 1839 by German scientists in the herb meadowsweet (Filipendula ulmaria), formerly known as Spiraea ulmaria. It is found naturally in white willow (Salix alba L.). As it can be isolated from nature, it is considered a valuable "natural product". In 1828, a small amount of salicin, which is a glucoside of salicyl alcohol, was isolated by the German scientist Johann Andreas Buchner. Salicin is one of the salicylates found not only in willow tree bark but in 36 other plants as well (Raskin, 1992; Hayat et al. , 2010). Synthetic SA also began its first commercial production in Germany in 1874.
In addition, SA plants are primarily found in the powdered crystalline form, melt at temperatures ranging from 157 to 159 °C and have a pH equal to 2.4. It may be expressed in all ways, whether glycosylated, methylated, or both. Salicylic acid (SA; o-hydroxybenzoic acid) has received broad interest from plant physiologists among the 21 molecules of signaling. Therefore, through its comprehensive signaling crosstalk with other growth hormones, SA has the capacity to control various aspects of plant life, especially in plant defense mechanisms, to different stresses (Horva'th et al. , 2007; Asensi-Fabado and Munne'-Bosch 2011; okotylo et al. , 2019).
SA is a cheap and environmentally sustainable plant growth regulator, in addition to its essential physiological functionality. Therefore, its use in boosting crop yield could be exploited under natural and stressful conditions. Other compounds known as secondary metabolites such as Jasmonic Acid (JA), Brassinosteroids (BRs), and Salicylic Acid (SA) also serve as growth regulators, in comparison to main growth regulators such as auxins (AUXs), cytokinins
(CKs), gibberellins (GAs), ethylene (ET) and abscisic acid (ABA), in addition to their primary protective function throughout plant pathogen infection (Figure 1). This secondary metabolite may be able to shield plants from biotic and abiotic stresses by activating a number of signaling pathways. Furthermore, SA has been shown to reduce the toxic effects of ROS created by salt stress by boosting antioxidant system activity (Belkadhi et al. , 2014; Mimouni et al. , 2016; Ma et al. , 2017). SA is involved in the control of many physiological processes, including increased metabolism of nitrogen, defense compound enzymes, and osmolytes inducing glycine betaine and proline in several different species (Khan et al. , 2010). In addition, the enhanced photosynthetic rate added by SA is correlated with increased activity of rubisco (Lee et al. , 2014) and absorption of K+ ion (s) (Fayez and Bazaid, 2014). Salicylic acid also greatly enhances soybean plant biomass under saline conditions (Farhangi-Abriz and Ghassemi-Golezani, 2018).
SA biosynthesis pathway
SA is synthesized along two distinct paths: the pathway of ammonia-lyase phenylalanine and the pathway of isochorism. The two processes take place in the cell at various sites, the first in the cytoplasm, while the second takes place in the cell's chloroplast. In the phenylalanine pathway, SA is synthesized from phenylalanine after a series of reactions. The enzyme called phenylalanine ammonia-lyase ( AL) plays a crucial role in the synthesis of secondary compounds such as salicylic acid, phenolic acid, and lignin along the phenylpropanoid pathway (Sorahinobar et al. , 2016). In the beginning, trans-cinnamic acid is produced from phenylalanine by the action of the enzyme phenylalanine ammonia-lyase ( AL). Trans-cinnamic acid is then converted to benzoic acid. The final step, the conversion of benzoic acid to SA is catalyzed by the enzyme known as 2benzoic-acid-2-hydroxylase (BA2H) (Horva'th et al. , 2007; Mustafa et al. , 2009).
As an intermediate product, SA is derived from chorismate via isochorismate in the Isochorismate pathway. Enzymes-isochorismate synthase (ICS) and isochorismate pyruvate lyase (I L) are involved in this two-step process (Verberne et al. , 2000; Strawn et al. ,
2007). The pathway of shikimic acid serves as a bridge between the digestion of carbohydrates and the biosynthesis of essential aromatic compounds such as tyrosine, phenylalanine, and tryptophan. In young pea plants, a similar result of the shikimate pathway in SA biosynthesis has been demonstrated (Szalai et al. 2011).
SA Pathway of signalling and transport
The transcriptional and posttranscriptional levels of the SA biosynthesis pathway are both regulated. MYBs (MY ELO BLAST; MYB 96 and MYB30), WRKYs (WRKY28 and WRKY46) and WI K (Wound-Induced Transcription Factors of Mitogen-Activated rotein Kinase) genes have been identified in plants at the transcriptional level. These genes positively regulate the isochorismate synthase pathway of ICS gene encoding, thus increasing SA production in plants (Vidhyasekaran, 2015). RNA binding proteins (RB ) have been reported to engage in several post-transcriptional processes at the post-transcriptional level. In specific, an RB from A. Thaliana, AtRB -defense-related (AtRB -DR1) has been found to influence SA biosynthesis through ICS1. For a variety of reasons, AtRB -DR1 mutant plants accumulated fewer SA and had higher SA overexpression lines than wild-type plants, and SID2 mRNA levels were higher overexpressor lines in AtRB -DR1 mutant plants (Qi et al. , 2010). After biosynthesis, SA can be freely transferred into cells, tissues, and organs (Kawano et al. 2004). A radio-tracer study of tobacco cell suspension culture revealed de novo activation of free SA secretion via the plasma membrane.
ROS and Ca2+tt-dependent (200 pM SA), and Ca2+tt-independent (20pM SA) transporters have regulated this secretion (Chen, 1999; Chen, et al., 2001). In soybeans, tonoplast ABC transporter-like protein-mediated SAG transfer into the vacuole (Dean and Mills 2004), whereas tonoplast antiporter was active in tobacco suspension culture cells (Dean, et al., 2005). While salicylic acid is not the mobile signal itself, SAR in other areas of the plant is still found to be formed. Furthermore, recent research suggests that the methyl ester, methyl salicylate, functions as a volatile SAR causing signal that is distributed to distant areas of the plant, including neighbouring plants (Shulaev, et al., 1997; Vlot, et al., 2008).
At low SA concentrations in plants, the enzyme salicylic acid carboxyl methyltransferase (16 µM) transforms SA to methyl salicylate (MeSA) (Dempsey et al. , 2011). Among the different forms of SA, only the methylated form (MeSA) has been shown to migrate nearby and systemically in plant tissue after pathogen infections (Seskar et al. , 1998). MeSA was therefore considered to be the long-distance signalling molecule through phloem translocation which moves from affected to unaffected leaves.
Physiological processes governed by SA under salinity stress
Seed germination
The seed germination regulation depends upon environmental factors and interactions between the plant hormones. The function of SA used in seed germination, other than naturally occurring phytohormones, has been controversial, as reports indicate that it can either impede germination or increase seed vigor. Furthermore, the role of SA in seed germination is affected by the genotype of the plant, the stress applied, and the experimental conditions (Lee and ark, 2010). Germination of Arabidopsis sid2 mutants missing ICS1-mediated SA biosynthesis was hypersensitive to salt stress (Lee et al. 2010). When the expression level of ICS1 was raised, the salt-induced germination inhibition was reversed (AlonsoRamirez, et al. , 2009).
The findings indicate that SA synthesis and accumulation are critical for seed germination, especially when salt stress is present. Interestingly, increased seed germination and seedling establishment in Arabidopsis under various abiotic stress conditions was noted when low 4 doses of SA were added (Rajjou et al. , 2006; Alonso-Ramı'rez et al. , 2009). Under salt stress (100150 mM NaCl) alone, 50 percent of seed germination was found in Arabidopsis, where 80 percent was reported in the presence of SA (0.05-0.5 mM) seed germination rate.
Interestingly, SA suggested regulation of ROS balance as a mechanism by which SA modulates germination during salt tension stress (Lee et al. 2010). Since SA and H2O2 form a "self-amplifying feedback loop" in response to numerous stresses (reviewed by Maheswari Jayakannan et al. , 2015). Furthermore, H2O2 causes the aggregation of SA, and SA increases the concentration of H2O2 (Shirasu et al. 1997; Harfouche et al. 2008). Hence, under salinity conditions, the germination promotion effect of SA is to minimize oxidative damage. In addition, the proteomic analysis showed that SA induces two superoxide dismutases in Arabidopsis germinating seeds, which could lead to increased antioxidant ability (Rajjou et al. , 2006). SA (0.5mM for 24 h) treatment often causes a major upregulation of protein transcription factors. Increasing seed germination by facilitating the synthesis of proteins required for seed germination as well as the mobilization or degradation of seed proteins acquired during seed maturation.
Exotic applications of three different levels of SA (0.25, 0.5, and 1mM) increased germination in Vicia faba under salinity stress (Anaya et al. , 2018). Salicylic acid treatment improved the germination rate of Coronilla varia seeds when they were exposed to drought stress by rising osmotic pressure (Ma et al. , 2017). The germination rate, percentage, and seed development of Dracocephalum moldavica seeds increase when they are primed with SA (ShaikhAbol-hasani et al. , 2019). SA applied exogenously to wheat increased germination indices (Fardus et al. , 2018). SA increased the germination parameters of coneflower plants (Darvizheh et al. , 2018). In soybeans, the application of SA resulted in the highest germination percentage (Aalam et al. , 2019).
Externally applied SA impact on growth and development
The effect of added SA varies depending on the plant species, growth level, and SA concentrations studied. The two findings of SA applied to plant physiological processes under optimal environmental conditions are observed in the contested studies. Several studies have documented that in different plant species, SA positively affects germination and/or development. For example, when the grains were subjected to pre-sowing seed-soaking treatment in SAA, improved 4germination and seedling growth were reported in wheat (Shakirova, 2007). Likewise, SA's aqueous solutions applied as a spray to soybean shoots significantly increased the growth of shoots, and especially roots also.
The 2nd characterization of Arabidopsis mutants with altered SA accumulation presented clear proof that SA was involved in plant development. cpr1/5/6 (constitutive expresser of R1/5/6), acd1/5/6/11 (accelerated cell death1/5/6/11), dnd1/2 (defence no death1/2), isd1 (lesions simulating disease1), nudt7 (nudix hydrolase7), agd2 (aberrant growth and death), and snc1 (suppressor of nudix hydrolase7) had higher biomass than wild type (reviewed in Miura et al. 2011; Rivas-San Vicente and lasencia 2011). The negatively regulated cell division and cell enlargement by SA have been proposed as a cause for the above growth variations (Xia et al. 2009; Hao et al. 2012). Similarly, 10nM, 100 μM-10mM SA treated soybean plants display increased shoot and root development, 20 percent and 45 percent, respectively, 7d after application. Shakirova et al. , (2003), noted that larger ears are produced by wheat seedlings treated with 50μM SA; increased cell division within the apical meristem of seedling roots.
Tomato seed pre-treatment with SA (150 ppm) improved root and shoot development under salinity tension (Ghoohestani et al. , 2012). Under salt tension, exogenous 4treatment of SA analogues like 2,6-dichloro-isonicotinic acid improved growth and production (Khan et al. , 2014). Under salt tension, foliar sprayed SA improved fenugreek plant growth, metabolism, and productivity (Babar et al. , 2014). Exogenous SA aids in the induction of plant growth and production, as well as the protection of plant membranes under heavy metal stress (Wang et al. , 2013; Yusuf et al. , 2013). SA (100–300 ppm) increased plant growth, specifically branching and biomass in Rosmarinus officinalis, according to El-Esawi et al. , (2017).
Effect on the rate of photosynthesis
Externally applied photosynthesis induced by SA is based on concentration and plant species (Ashraf et al., 2010). In general, SA increased the growth rate at low concentrations (less than 100 μM) and decreased growth at moderately high concentrations (>1 mM) in different plant species (Rivas-San Vicente and lasencia 2011). Furthermore, low concentrations of SA (less than 10 M) increased salt-induced photosynthesis in many plant species by increasing carbon fixation, transpiration, stomatal conductance, and antioxidant activity (Stevens et al., 2006; Szepesi et al., 2008; Nazar et al., 2011; oo'r et al., 2011a). According to Lotfi et al., (2020), SA at (1.5mM) had a lower impact on improving photosynthetic rate than at the optimum stage of SA (1.0 mM).
Data from several studies indicates that SA is an essential photosynthesis regulator because it affects the structure of leaves and chloroplasts (Uzunova and opova, 2000), the closure of stomata (Melotto et al. , 2006), the content of chlorophyll and carotenoids (Chandra and Bhatt, 1998; Fariduddin et al. , 2003) and the activity of enzymes such as RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) and carbon carboxylase/oxygenase ( ancheva and opova, 1998; Slaymaker et al. , 2002). Tahjib-Ul-Arif et al. , (2018) observed SA elevated photosynthetic rate in maize by facilitating carboxylation rate, chlorophyll amount (S AD values), and increasing turgor. When SA doses were augmented to increase the chlorophyll content in mustard, lemongrass, and mung bean plants under stress conditions, similar findings were observed (Idrees et al. , 2010; Zaid et al. , 2019; Lotfi et al. , 2020).
In Satsuma mandarin leaves, exogenous SA prevents the photosynthetic apparatus from photo damage caused by intense light. This defensive effect was related to several protective mechanisms. For example, exogenous SA increased the electron transport rate and quantum yield of SII, as well as the relative contents of D1 protein and Deg1 protease, both of which were induced by synergetic factors such as high temperature and light (Qiu et al., 2011). SA pretreatment in wheat not only improved the activity of protein kinase, but it also decreased the degradation of D1 protein during the heat and high light stress, and it also accelerated the recovery of D1 protein levels until the stress was removed. These findings show that SA pre-treatment of wheat leaves will greatly reduce SII damage caused by heat and high light tension, speeding up photosynthesis restoration (Zhao et al. 2011). When exposed to chilling conditions, SA application results in improved photosynthesis in Dendrobium officinale. The consistency of SII and D1 proteins has increased, although the stability of the chloroplast membrane has been maintained (Huang et al., 2016).
SA mediated Antioxidant Activity
ROS function as a secondary messenger and participate in several developmental and defence responses to stresses in plants at optimal levels (Baxter et al. , 2013; Czarnocka and Karpiski, 2018; Farooq et al. , 2019). However, they tend to disrupt cell metabolism and other physiological processes in crop plants as their abundance increases under stress conditions. In general, plants stimulate their inborn resistance mechanisms, such as antioxidant enzymes to maintain a balance between ROS generation and quenching. Excess ROS have been shown to have lethal effects on tissues that have been exposed to salt stress (Foyer et al. 2017). SOD, CAT, A X, and G X are among the main enzymatic antioxidant resistance mechanisms in plants (Sharma et al. , 2012; Mekawy et al. , 2018b; Yassin et al. , 2019).
Recent research has found a correlation between ROS accumulation and SA acid-mediated defence signalling in response to various stresses (Brosché et al. , 2014; Xu and Brosché, 2014; Nazar et al. , 2017). Application of SA enhanced the activity of catalase (CAT), peroxidase ( OX), and superoxide dismutase (SOD) in mung bean under aluminum (Al) stress (Ali, 2017), in soybean under arsenic stress (Chandrakar et al. , 2016), and in maize under chromium stress (Islam et al. , 2016). In Arachis hypogeal cultivars, osmotic stress was created when applying an herbicide known as Basagran®. However, SA application enhanced the content of total phenols, sugars, and the activity of enzymatic antioxidants and regulates osmotic pressure. These findings reflect SA's protective function in the detoxification of Basagran® (Radwan et al. , 2019).
The synthesis of non-enzymatic antioxidants such as proline, glycine betaine, glutathione, ascorbic acid, and carotenoids is also induced by SA. Under NaCl stress, SA increased glutathione, ascorbate, dehydroascorbate, and oxidized glutathione levels, which act as antioxidants and minimise hydrogen peroxide levels (Yan et al., 2018). Under salinity, supplementing with SA improved glycine betaine, proline, and carbohydrates, resulting in osmo-protection and ionic equilibrium (Farhangi-Abriz and Ghassemi-Golezani, 2018). Externally applied SA increases the rate of SOD, CAT, A X, and DHAR and GR operation, as well as the content of proline, GB, and sugars in Vigna angularis under salt stress (Ahanger et al., 2020). Similarly, Singh and Tripathi (2019), found that SA application helps plants resist osmotic pressure by growing sugar levels and rendering them salt resistant.
SA's flowering-inducing action
The role of SA in flowering control has long been recognized. Initially, it was discovered that 4µM SA encourages the development of flower buds from tobacco callus (Lee and Skoog, 1965). SA later identified the phloem-transmittable factor secreted in aphid honeydew. It was responsible for flowering in Lemna gibba plants that were subjected to a non-photo-inductive light cycle (Cleland and Ajami, 1974). Flowering is also induced by SA (3-10 M) in the Lemnaceae family, including long day (LD), short day (SD), and photoperiod-insensitive types (Khurana and Cleland, 1992). Experiments have shown that thermogenic plant inflorescences produce higher levels of endogenous SA (Raskin et al. , 1990). SA levels in the leaves of non-thermogenic plants like tobacco and Arabidopsis increase 5-fold and 2-fold, respectively, at the beginning of or during the transition to flowering (Yalpani et al. , 1993; Abreu and Munne- Bosch, 2009).
Aghdam et al., (2016) discovered that SA treatment improved flowering reduced stress, and extended the post-harvest period. According to Wada et al., (2014), SA increased flowering in harbitis zero under nutrientdeficient conditions. Yamada and Takeno, (2014) discovered that treating harbitis zero or Japanese morning glory with SA improved flowering by increasing expression of the nFT2 gene in nutritionally deficient conditions. Continuous SA exposure on Gladiolus flowers improved flowering, blooming rate, vase survival, and thus delayed senescence by reducing ion leakage in petals (Hatamzadeh et al., 2012). After applying various doses of SA, the number of female flowers 3in cucumbers was also increased (Sultan et al., 2016).
A crucial connection between SA and flowering has recently been discovered in sunflowers. When expressed constitutively in Arabidopsis, the transcription factor HAHB10, which belongs to the HD-Zip II family, induces flowering by upregulating specific flowering transfer genes and repressing genes linked to biotic stress. Surprisingly, expression of HAHB10 is caused by SA therapy and infection with . syringae (Dezar et al. , 2011). Flowering in Arabidopsis is regulated by a complex network of pathways, and the role of several genes has been identified. CONSTANS regulates the photoperiod pathway (CO). FLOWERING LOCUS C (FLC) is a flowering repressor gene that combines the autonomous and vernalization pathways. Two integrators, FLOWERING LOCUS T (FT) and SU RESSOR OF OVEREX RESSION OF CONSTANS 1, converge on a limited number of integrators (SOC1). The photoperiod and autonomous pathways are related by the SOC1 gene, which encodes a MADS-box protein that is regulated by CO and suppressed by FLC (Mouradov et al. , 2002). Latest studies have shown that SA is active in controlling these genes' transcription (Martı'nez et al. , 2004).
SenescenceSA requirement for senescence regulation
In addition to the significant role of SA in cell redox homeostasis and photosynthesis, the Senescence SA necessity for senescence control is also involved in senescence regulation. Senescence is characterized by a decrease in photosynthetic activity due to a lack of antioxidant ability and increased ROS levels. Senescence is characterized by significant changes in gene expression, and SA plays a significant role in this. Several SAG transcripts, such as SAG12, are significantly reduced in SA-deficient Arabidopsis plants (Morris et al., 2000). In Arabidopsis, SA also promotes the expression of senescence-related genes. The genes involved are aV E, cV E, WRKY6, WRKY53, and SEN1. They encode two vacuolar synthesis enzymes, two transcription factors, and a protease (Robatzek and Somssich, 2001; Miao et al., 2004; Schenk et al., 2005).
Autophagy induction by SA during developmental leaf senescence
The induction of autophagy by SA during developmental leaf senescence is critical for plant growth, particularly during senescence and the defense response (Kwon and ark, 2008). The role of autophagy in the senescence process was discovered by analyzing Arabidopsis knock-out plants affected in different ATG (autophagy) genes. In revised literature, Bassham et al. ,
(2006) reported that under nutrient-rich conditions, some plants display an improved senescence phenotype. In senescent tissues, the autophagy genes ATG5, ATG8, and ATG12 are strongly expressed (Wagstaff et al. , 2009). Autophagy, which is induced by SA, regulates an acyl-CoA-binding protein (ACB 3), which binds phosphatidylcholine and phosphatidylethanolamine. Interfering with the development of the ATG5– phosphatidylethanolamine complex and, as a result, disrupting the formation of autophagosomes and subsequent degradation of ATG8 (Xiao and Chye, 2010; Xiao et al. , 2010).
Table 1. Summary on the role of exogenous applied salicylic acid in plants when subjected to NaCl stress
Salt stress NaCl Dose (s) |
Plant |
Concentration (SA) |
SA induced effect |
Reference (s) |
1.2, 4, 8 and 12 dS m-1 |
Vigna radiata |
100, 200 and300 mg L-1 |
Improved growth and productivity by enhanced nitrogen metabolism by increasing NRA, NiRA, & other proteins |
Akhtar et al. , (2013) |
90, 120, 150 and 200 mM |
Vicia faba |
0.25, 0.5 and 1 mM |
Seed priming with SA improved and enhanced germination traits under various stress levels |
Anaya et al. , (2018) |
2-12 dS m -1 |
Hordeum vulgare |
0.5, 1.0, 1.5, and 2.0 mM |
Induced Salinity Tolerance through Manipulation of Ion Distribution Rather than Ion Accumulation in barley plants |
Pirasteh-Anosheh et al. , (2017) |
40 and 80mM |
Egletes viscosa |
1.0 mM |
modulated primary and volatile metabolites to alleviate salt stress induced photosynthesis impairment. |
Batista et al., (2019) |
100 and 200 mM |
Raphanus sativus |
2 and 5mM |
SA Induced Photosynthetic Adaptability of Raphanus sativus to Salt Stress by enhanced Antioxidant acitvity |
Bukhat et al. , (2020) |
3, 6 and 9 dS m -1 |
Vigna radiata |
0.5, 1, and 1.5 mM |
Regulated photosynthetic electron transfer and stomatal conductance of mung bean under salinity stress |
Lotfi et al. , (2020) |
50 mM NaCl |
Vigna radiata |
0.5 mM |
Salicylic Acid and Sulphur- Enhanced Defence Systems, Nitrogen Metabolism, Photosynthetic, and Growth Potential of Mungbean (Vigna radiata) Under Salt |
Hussain et al. , (2020) |
Stress
Cross-talks with other phytohormones
By cross-talking with auxins, gibberellins, abscisic acid, jasmonic acid, and ethylene hormones, SA plays a diversified role in plant growth processes and hence minimizes stressful situations (Khan et al. , 2014; Zhang and Li, 2019; Arif, et al. , 2020).
The discovery that the transcription factors OB 1, OB 2, and OB 3 of SA-inducible DOF are also receptive to AUXs (Kang and Singh, 2000) and provides a clear link between the signalling pathways of SA and AUX. Wheat seedlings treated with 50 M SA showed a higher rate of cell division. It is associated with an increase in endogenous AUX indole acetic acid levels (Shakirova et al., 2003). The Arabidopsis cpr5, cpr6, and snc1 mutants, which have decreased apical dominance and stunted growth phenotypes similar to AUX deficient plants, have increased endogenous SA levels. This suggests interaction caused by SA with the AUX-mediated responses. In addition, Tryptophan, an IAA biosynthetic pathway intermediate, is implicated in SA-induced pathogenic resistance (Denancé et al., 2013).
In general, during the development of structural acquired resistance, SA is antagonistic to ABA (SAR). ABA's exogenous application hampered SAR induction, while SAR activation by SA suppressed ABA signalling by ABA (Yasuda et al. , 2008). Furthermore, high endogenous SA concentrations improved salt tolerance in two ABA-sensitive mutants (aba3 and siz1) (Asensi-Fabado and Munne-Bosch, 2011; Miura et al. , 2011), suggesting that ABA signalling suppression is necessary for salt tolerance. Exogenous gibberellins added under NaCl (150 mM) stress slightly improved SA-deficient sid mutant germination (AlonsoRamirez et al. , 2009), implying that gibberellins would compensate for SA deficiency.
SA prevented the decrease of auxin and cytokinin in wheat to promote growth while retaining high levels of ABA to boost the tolerance of plant salt (Shakirova, et al. , 2003). SA treatment downregulated ABA biosynthesis genes in Limonium bicolor, while upregulated Ka biosynthesis genes increased seed germination by preserving a favorable GA/ABA equilibrium under saline tension (Liu et al. , 2019). It indicates the resistance of SA regulated plant salt resistance by interactions with other plant hormones.
CONCLUSION
This analysis concludes that SA functions as a potent phytohormone when used against environmental signals. Beyond the defensive response in plant immunity, it controls a number of plant growth production responses, especially at low concentrations. It promotes seed germination, photosynthesis, plant growth and production, and flowering, among other physiological processes. It also improves the activity of ROS-scavenging enzymes. Thus, cellular homeostasis is maintained and salinity stress tolerance is provided.
Further studies are needed to establish molecular and signalling pathways of abiotic stress tolerance due to the role of SA in abiotic stress management.
ACKNOWLEDGEMENTS
The first author is thankful to DST URSE-II, New Delhi, for the financial support and also to Head, Department of Botany, Annamalai university for providing an internet facility to carry out this work.
CONFLICTS OF INTEREST
All authors have declared that they do not have any conflict of interest for publishing this research.
Список литературы Salicylic Acid and Salt Stress Tolerance in Plants: A Review
- Aalam L., Sedghi M., and Sofalian O. (2019) Sodium nitroprusside and salicylic acid decrease antioxidant enzymes activity in soybean. J. Plant Physiol. 10 (1), 3073–3077.
- Abogadallah G.M. (2010) Antioxidative defense under salt stress. Plant Signal. Behav. 5 (4), 369–374.
- Abreu M.E., and Munne´ -Bosch S. (2009) Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana. J. exp. Bot., 60, 1261–1271.
- Aghdam M.S., Jannatizadeh A., Sheikh-Assadi M., and Malekzadeh P. (2016) Alleviation of postharvest chilling injury in anthurium cut flowers by salicylic acid treatment. Sci. Hortic., 202, 70–76.
- Ahanger M.A., Aziz U., Alsahli A.A., Alyemeni M.N., and Ahmad P. (2020) Influence of exogenous salicylic acid and nitric oxide on growth, photosynthesis, and ascorbate glutathione cycle in salt stressed Vigna angularis. Biomolecules, 10(1), 42.
- Ahmad P., Alyemeni M.N., Ahanger M.A., Egamberdieva D., Wijaya L., and Alam P. (2018) Salicylic acid (SA) induced alterations in growth, biochemical attributes and antioxidant enzyme activity in faba bean (Vicia faba L.) seedlings under NaCl toxicity. Russ. J. Plant Physiol., 65, 104–114.
- Akhtar J., Ahmad R., Ashraf M. Y., Tanveer A., Waraich E. A., and Oraby H. (2013) Influence of exogenous application of salicylic acid on salt-stressed mungbean (Vigna radiata L.): growth and nitrogen metabolism. Pak. J. Bot., 45(1), 119-125.
- Ali B. (2017). Salicylic acid induced antioxidant system enhances the tolerence to aluminium in mung bean (Vigna radiata L. Wilczek) plants. Indian J. Plant Physiol., 22, 178–189.
- Alonso-Ramirez A., Rodriguez D., Reyes D., Jimenez J.A., Nicolas G., Lopez-Climent M., Gomez-Cadenas A., and Nicolas C. (2009) Evidence for a role of gibberellins in salicylic acid-modulate early plant responses to abiotic stress in Arabidopsis seeds. J. Plant Physiol., 150:1335–1344.
- Anaya F., Fghire R., Wahbi S., and Loutfi K. (2018) Influence of salicylic acid on seed germination of Vicia faba L. under salt stress. J. Saudi Soc. Agric. Sci., 17, 1–8.
- Arif Y., Sami F., Siddiqui H., Bajguz A., and Hayat S. (2020) Salicylic acid in relation to other phytohormones in plant: a study towards physiology and signal transduction under challenging environment. Environ. Exp. Bot., 104040.
- Asada K. (1992) Ascorbate peroxidase, a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant., 85: 235–41.
- Asada K. (1999) The water-water cycle in chloroplasts: scavenging of active oxygens and of excess photons. Ann. Rev. Plant physiol. Mol. Boil., 50: 601-639.
- Asensi-Fabado M., and Munne´-Bosch S. (2011) The aba3-1 mutant of Arabidopsis thaliana withstands moderate doses of salt stress by modulating leaf growth and salicylic acid levels. Plant Growth Regul., 30:456–466.
- Ashraf M., Akram N.A., Arteca R.N., and Foolad M.R. (2010) The physiological, biochemical and molecular roles of brassinosteroids and salicylic acid in plant processes and salt tolerance. Crit Rev Plant Sci., 29:162–190.
- Babar S., Siddiqi E.H., Hussain I., Hayat Bhatti K., and Rasheed R. (2014) Mitigating the effects of salinity by foliar application of salicylic acid in fenugreek. Physiol. J. 1–6.
- Bassham D.C., Laporte M., Marty F., Moriyasu Y., Ohsumi Y., Olsen L.J., and Yoshimoto K. (2006) Autophagy in development and stress responses of plants. Autophagy, 2, 2–11.
- Batista V.C.V., Pereira I.M.C., de Oliveira Paula-Marinho S., Canuto K.M., Pereira R.D.C.A., Rodrigues T.H.S., ... and de Carvalho H.H. (2019) Salicylic acid modulates primary and volatile metabolites to alleviate salt stress-induced photosynthesis impairment on medicinal plant Egletes viscosa. Environ. Exp. Bot., 167, 103870.
- Baxter A., Mittler R., and Suzuki N. (2013) ROS as key players in plant stress signalling. J. exp. Bot., 65, 1229–1240.
- Belkadhi A., De Haro A., Soengas P., Obregon S., Cartea M.E., Chaibi W., and Djebali W. (2014) Salicylic acid increases tolerance to oxidative stress induced by hydrogen peroxide accumulation in leaves of cadmium-exposed flax (Linum usitatissimum L.). J. Plant Interact., 9, 647–654.
- Borsani O., Valpuesta V., and Botella A. (2001) Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol., 126, 1024–1030.
- Brosché M., Blomster T., Salojärvi J., Cui F., Sipari N., Leppälä J., …… and Kangasjärvi J. (2014) Transcriptomics and functional genomics of ROSinduced cell death regulation by RADICALINDUCED CELL DEATH1. PLoS Genet., 10, e1004112.
- Bukhat S., Manzoor H., Zafar Z.U., Azeem F., and Rasul S. (2020). Salicylic acid induced photosynthetic adaptability of raphanus sativus to salt Stress is associated with antioxidant capacity. Plant Growth Regul., 39(2), 809-822.
- Chandra A., and Bhatt R.K. (1998) Biochemical and physiological response to salicylic acid in relation to the systemic acquired resistance. Photosynthetica, 35, 255–258.
- Chandrakar V., Dubey A., and Keshavkant S. (2016) Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L. J. Soil Sci. Plant Nutr., 16.
- Chen H.J. (1999) Ca2+ dependent excretion of salicylic acid in tobacco cell suspension culture. Bot Bull Acad Sin., 40:267–273.
- Chen H-J., Hou W-C., Kuc J., and Lin Y-H. (2001) Ca2+ dependent and Ca2+ independent excretion modes of salicylic acid in tobacco cell suspension culture. J Exp. Bot., 52:1219–1226.
- Chini A., Grant J.J., Seki M., Shinozaki K., and Loake G.J. (2004) Drought tolerance established by enhanced expression of the CCI-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1. Plant J., 38, 810–822.
- Chinnusamy V., and Zhu J.K. (2003) Plant salt tolerance. Plant responses to abiotic stress 2(1): 241-270.
- Cleland C.F., and Ajami A. (1974) Identification of the flower-inducing factor isolated from aphid honeydew as being salicylic acid. Plant Physiol., 54, 904–906.
- Cortleven A., Leuendorf J.E., Frank M., Pezzetta D., Bolt S., and Schmülling T. (2019) Cytokinin action in response to abiotic and biotic stresses in plants. Plant Cell Environ. 42(3), 998-1018.
- Czarnocka W., and Karpiński S. (2018) Friend or foe? Reactive oxygen species production, scavenging and signaling in plant response to environmental stresses. Free Radical Biol. Med., 122, 4–20.
- Darvizheh H., Zahedi M., Abbaszadeh B., and Razmjoo J. (2018) Effects of foliar application of salicylic acid and spermine on maternal plant under drought stress on germination indices of purple coneflower (Echinacea purpurea). Iran. J. Seed Res., 5, 1–19.
- De Lourdes Oliveira Otoch M., A.C.M.S., Farias De Aragao, M.S., M. Da Guia Silva Lima, E.G.O., and FernandesDeMelo, D. (2001) SaltmodulationofvacuolarH+-ATPase and H+- pyrophosphatase activities in Vigna unguiculata. J. Plant Physiol., 158 (5), 545–551.
- Dean J.V., and Mills J.D. (2004) Uptake of salicylic acid 2-O-b-D-glucose into soybean tonoplast vesicles by an ATP-binding cassette transporter-type mechanism. J. Plant Physiol.,120:603–612
- Dean J.V., Mohammed L.A., and Fitzpatrick T. (2005) The formation, vacuolar localization, and tonoplast transport of salicylic acid glucose conjugates in tobacco cell suspension cultures. Planta, 221:287–296.
- Debnath M., Ashwath N., Hill C.B., Callahan D.L., Dias D.A., Jayasinghe N.S., Midmore D.J., and Roessner U. (2018) Comparative metabolic and ionomic profiling of two cultivars of Stevia rebaudiana Bert. (Bertoni) grown under salinity stress. Plant Physiol. Biochem., 129, 56–70.
- Dempsey D.M.A., Vlot A.C., Wildermuth C.M., and Klessig F.D. (2011) Salicylic acid biosynthesis and metabolism. The Arabidopsis Book, 9: e0156.
- Denancé N., Sánchez-Vallet A., Goffner D., and Molina A. (2013) Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front. Plant Sci., 4, 155.
- Dezar C.A., Giacomelli J.I., Manavella P.A., Re D.A., Alves-Ferreira M., Baldwin I.T., Bonaventure G., and Chan R.L. (2011) HAHB10, a sunflower HD-Zip II transcription factor, participates in the induction of flowering and in the control of phytohormonemediated responses to biotic stress. J. Exp. Bot., 62, 1061–1076.
- El-Esawi M.A., Elansary H.O., El-Shanhorey N.A., Abdel-Hamid A.M.E., Ali H.M., and Elshikh M.S. (2017) Salicylic acid-regulated antioxidant mechanisms and gene expression enhance rosemary performance under saline conditions. Front. Physiol., 8, 716.
- El-Ramady H., Alshaal T., Bakr N., Elbana T., Mohamed E., and Belal A.A. (2019). Soils of Egypt. In: World Soils Book Series, first ed. Springer International Publishing AG. https://doi.org/10.1007/978-3-319-95516-2.
- FAO and ITPS (2015) Status of the World's Soil Resources (SWSR) – Main Report. Food and Agriculture Organization of the United Nations and Intergovernmental Technical Panel on Soils, Rome, Italy.