СБОР ГЕНЕТИЧЕСКИХ БИОМАТЕРИАЛОВ (ДНК) С ПОВЕРХНОСТЕЙ: МЕТОДЫ И УСТРОЙСТВА

Д. Г. Петров; Е. Д. Макарова; И. Е. Антифеев; М. В. Зайцева; D. G. Petrov; E. D. Makarova; I. E. Antifeev; M. V. Zaitseva

Научные статьи \ Математика. Естественные науки \ Химия. Кристаллография. Минералогия \ Аналитическая химия

СБОР ГЕНЕТИЧЕСКИХ БИОМАТЕРИАЛОВ (ДНК) С ПОВЕРХНОСТЕЙ: МЕТОДЫ И УСТРОЙСТВА

Автор: Д. Г. Петров, Е. Д. Макарова, И. Е. Антифеев, М. В. Зайцева

Журнал: Научное приборостроение @nauchnoe-priborostroenie

Рубрика: Приборостроение физико-химической биологии

Статья в выпуске: 1, 2024 года.

Бесплатный доступ

В обзоре рассмотрены современные технологии и тенденции развития в разработке приборов для взятия проб, в том числе контактной ДНК с различных поверхностей и предметов. В статье рассматривается эффективность сенсорных методов восстановления ДНК и устройств, используемых для сбора ДНК с различных поверхностей на месте преступления для судебно-медицинской лаборатории, а также рассмотрены проблемы, ограничения методов и современные тенденции развития. Разобраны распространенные и новые методы сбора проб, а именно: соскабливание, вырезание, тампоны (и протирание тампонами), подтяжка лентой и гелем, замачивание, Bardole M-vac, сухой и влажный вакуум и другие.

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ДНК-судебная экспертиза, контактная ДНК, методы извлечения ДНК, сбор биоматериалов с поверхности, тампоны, протирание тампонами, лифтинг клейкой лентой, лифтинг гелями, мокрое вакуумирование, сухое вакуумирование, метод погружения

Короткий адрес: https://sciup.org/142240145

IDR: 142240145 | УДК: 543.054, 543.062

COLLECTING GENETIC BIOMATERIAL (DNA) FROM SURFACES: METHODS AND DEVICES

The review survey the evolution and current progress in designing instrumentation for sampling touch (contact) DNA and biological stains on the variety of item surfaces. The paper considers the effectivity of touch (contact) DNA recovery methods and devices being use for DNA collection from various surfaces at the crime scene and in the forensic laboratory as well as the explored challenges, limitations and current trends. The discussion covers earlier and novel tools, namely: scraping, cutting-out, swabs (and swabbing), tape and gel lifting, soaking, the Bardole M-VAC, dry and wet vacuum and the others.

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