Seasonality of androgenetic responses in the anther culture in vitro in rice (Oryza sativa L.)

The conditions for growing donor plants in androgegesis in vitro are considered from the point of view of the influence of physical factors on the plants (illumination, length of daylight, temperature, nutrition of plants). A priori it is believed that any period of the year (season) is suitable for plant tissue culture in vitro, and this is the main advantage of in vitro technologies compared to traditional ones. Seasonality is taken into account only when comparing donor plants grown in the field and under controlled conditions. However, we have not found reports on the study of using procedure of anther in vitro culture throughout the year. This paper is the first to show that, under uniform conditions of donor plants growing, the frequency of callus formation and regeneration of rice ( Oryza sativa L.) plants from anthers in vitro culture differs depending on the month (season) when explants were collected. The aim of the studies was to study the seasonal dependence of in vitro androgenetic responses of O...