Usage of DNA markers for screening and identification of sunflower genotypes of hybrid origin on AHAS1 gene

A molecular genetic study of sunflower F1 and F2 hybrids on a microsatellite locus located within the mutant AHAS1 gene associated with herbicide resistance was performed. The aim of the work was to screen F1 and F2 sunflower hybrid populations with usage of DNA markers and identification of homozygous segregants containing the gene for resistance to SU herbicides. The possibility of identification of hybrid plants with different alleles of the AHAS1 gene was shown. The efficiency of the allele of 191 b.p. in the homo- and heterozygous state for marker selection of genotypes resistant to the herbicide of the sulfonylurea group was confirmed. F2 plants that are carriers of this gene in the homozygous state can be used as an initial germplasm in breeding for the purpose of creating new inbred lines with genetically determined resistance to SU herbicides.