Small HSPs molecular weights as new indication to the hypothesis of segregated status of thermophilic relict Gmelinoides fasciatus among Baikal and palearctic amphipods
Автор: Protopopova M.V., Takhteev V.V., Shatilina Zh.M., Pavlichenko V.V., Axenov-gribanov D.V., Bedulina D.S., Timofeyev M.A.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.7, 2011 года.
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Among the great diverse of Baikal fauna of amphipods (more than 350 species and subspecies) Gmelinoides fasciatus (Stebbing 1899) takes the special place. It is characterized by a high morphological variability and wide thermal-adaptive possibilities. By common opinion G. fasciatus is a thermophilic relict segregated from other Baikal temperature sensitive amphipods' fauna. In present study we tested hypothesis of segregated status of G. fasciatus among some Baikal and some Palearctic amphipods using of heat-shock proteins' (HSP) molecular weight as comparative biomarkers. We used heat-shock proteins (HSP) from two families: HSP70 and small HSP (sHSP) immunochemically related to α-crystalline. 15 species of Baikal amphipods from different genera and families and 2 Palearctic species from genus Gammarus were tested. It was shown, that molecular weights of HSP70 were the same in all investigated species and corresponds close to 70 kD. In the contrast, the molecular weights of sHSP in G. fasciatus was 37 kD and differed from all other species, in which molecular weights of sHSP were 35 kD. In sum, this study showed that sHSPs molecular weights may relate to evolutional differences between the close related species. Additionally, obtained data can be taken as new indication of segregated status of thermophilic relict G. fasciatus which linked with its phylogenetic history in Lake Baikal.
Amphipods, baikal, endemic, relict, heat-shock proteins
Короткий адрес: https://sciup.org/14323521
IDR: 14323521
Список литературы Small HSPs molecular weights as new indication to the hypothesis of segregated status of thermophilic relict Gmelinoides fasciatus among Baikal and palearctic amphipods
- Arrigo, A.P., Muller, W.E.G. (Eds.) (2002) Small Stress Proteins. Series: Progress in Molecular and Subcellular Biology.
- Bazikalova, A.Y. (1945) Amphipods of Lake Baikal. Proceeding of Baikal limnological station, 11, 1-440 (in Russian).
- Bers, G., Garfin, D. (1985) Protein and nucleic add blotting and immunobiochemical detection. BioTechniques, 3, 276-288.
- Boorstein, W.R., Ziegelhoffer, T., Craig, E.A. (1994) Molecular evolution of the HSP70 multigene family. J. Mol. Biol., 38, 1-17.
- Dorogostayskiy, V.Ch. (1923) Vertical and horizontal distribution of fauna of Lake Baikal. Proceeding of prof. and lecturer of Irkutsk state university, 4, 103-131 (in Russian).
- Gomanenko, G.V., Kamaltynov, R.M., Kuzmenkova, Zh.V., Berenos, K., Scnerbakov, D.U. (2005) Structure of baikalian amphipods Gmelinoides fasciatus (Stebbing) population. Genetic, 41, 1108-1114.
- Florensov, N.A. (Ed) (1977) Limnology of littoral zone of Lake Baikal. Novosibirsk, Nauka (in Russian).
- Lindquist, S. (1986) The heat-shock response. Annu. Rev. Biochem., 55, 1151-1191.
- Matafonov, D.I., Itigilova, M.Ts., Kamaltynov, R.M., Faleychik, L.M. (2005) Baikal endemic species Gmelinoides fasciatus (Micruropodidae, Gammaroidea, Amphipoda) in lake Arahley. Zoologicheskii Zhurnal, 84, 321-329 (in Russian).
- Mats, V.D. Tscerbakov, D.U. (2008) Geological development of Baikal region and formation unique biodiversity of Baikal. Development of life in process abiotic changes of the Earth: Proceeding scientific-practical conference (s. Listvyanka Irkutskaya oblast, 18-20 of March 2008). Novosibirsk: Publishers of Siberian department of academia of science, pp. 155-175 (in Russian).
- Pleshanov, A.S., Takhteyev, V.V. (2008) Refuge of baikalian Siberia as reservation of unique biodiversity. Development of life in process abiotic changes on the Earth: Proceeding scientific-practical conference (s. Listvyanka Irkutskaya oblast, 18-20 of March 2008). Novosibirsk: Publishers of Siberian department of academia of science, pp. 358-370 (in Russian).
- Ravaux, J., Toullec, J.Y., Leger, N., Lopez, P., Gaill, F., Shillito, B. (2007). First hsp70 from two hydrothermal vent shrimps, Mirocaris fortunata and Rimicaris exoculata: Characterization and sequence analysis. Gene, 386, 162-172.
- Sanders, B.M. (1993) Stress proteins in aquatic organisms: an environmental perspective. Crit. Rev. Toxicol., 23, 49-75.
- Segu-Simarro, J.M., Testillano, P.S., Risueno, M.C. (2003) Hsp70 and Hsp90 change their expression and subcellular localization after microspore embryogenesis induction in Brassica napus L. J. Struct. Biol., 142, 379-391.
- Takhteev, V.V. (2000) Essay about amphipods of Lake Baikal (taxonomy, comparative ecology, evolution). Irkutsk: Publishers of Irkutsk State University. (in Russian).
- Takhteev, V.V. (2009) Amphipods (Amphipoda) of thermal and mineral springs of northern part Baikal region. Biota of waterbodies of Baikal rift zone. Irkutsk: Publishers of Irkutsk state university, 123-130 (in Russian).
- Timofeyev, M.A. Kirichenko, K.A. (2004) Experimental evaluation abiotic factors role for limitation spreading of endemics from Lake Baikal in example of amphipods. Siberian ecological journal, 1, 41-50 (in Rusian).
- Timofeyev, M.A., Shatilina, Z.M., Bedulina, D.S., Protopopova, M.V., Pavlichenko, V., Grabelnich, O.I., Kolesnichenko, A.V. (2008) Evaluation of biochemical responses in Palearctic and Lake Baikal endemic amphipod species exposed to CdCl2. Ecotox. Environ. Safe., 70, 99-105.
- Timofeyev, M.A., Shatilina, Zh.M., Protopopova, M.V., Bedulina, D.S., Pavlichenko, V.V., Kolesnichenko, A.V., Steinberg, C.E.W. (2009) Thermal stress defense in freshwater amphipods from contrasting habitats with emphasis on small heat shock proteins (sHSPs). J. Therm. Biol., 34, 281-285
