The content of dien conjugates and shiff bases in Neonothopanus nambi myccelium at different levels of its luminescence in stress conditions

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The ratio of the intensity of light emission from Neonothopanus nambi mycelium under stress and content in the biomass of the fungus of primary and final products of lipid peroxidation (PLP) - diene conjugates (DC) and Schiff bases (SB) was stud- ied. For this purpose the samples of mechanically damaged mycelium was incubated in nutrient pota- to and saccharose medium without any additives (control) and in the conditions of influence of addi- tional stressful factors (experience): an additive on the medium of inhibitor of a mushroom lumines- cence (salicylic acid (SA) in concentration of 0.5 mm), acidulations of the environment to pH 5.0 and4.0 additives of acetic acid. The content of studied PLP was evaluated in biomass of control and test samples of mycelium after 4 hours of incubation (registering maximum intensity of fungal lumines- cence). DC from biomass of a mycelium extracted by the mix of solvents heptane - isopropanol with subsequent division of phases. DC content in hep- tane phase and isopropanol phase, reflecting the intensity of peroxidation in the fungus of neutral lipids and phospholipids, respectively, was deter- mined spectrophotometrically using optical density of the samples at 232 nm...

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Luminous fungi, diene conjugates, schiff bases, reactive oxygen forms

Короткий адрес: https://sciup.org/140243399

IDR: 140243399

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