Conservation of leucocytes in the conditions of cryoanabiosis (-40°C)

Trying to meet the requirements in leukocytes the clinicists are facing the pressing problem of conservation of cells in a functionally valid condition which is possible in deep cryoanabiosis (-196°C). This conservation method is not, for the number of reasons, available for the purpose of a wide use. The aim of the present was development of an efficient, readily available and economical method for conservation of leukocytes at a moderately low temperature of -40°C. It was shown that use of this method allows for conservation of blood nuclear cells in a functionally valid condition for a period of 30 days.