Solvent/non-solvent treatment as a method for surface coating of poly(-caprolactone) 3D-printed scaffolds with hydroxyapatite
Автор: Bocharov V.S., Dubinenko G.E., Popkov D.A., Popkov A.V., Tverdokhlebov S.I.
Журнал: Гений ортопедии @geniy-ortopedii
Рубрика: Оригинальные статьи
Статья в выпуске: 6 т.29, 2023 года.
Бесплатный доступ
Introduction Over the last decades numerous new materials and techniques for bone tissue engineering have been developed. The use of bioresorbable polymeric scaffolds is one of the most promising techniques for surgical management of bone defects. However, the lack of bioactive properties of biodegradable polymers restricts the area of their application for bone tissue engineering.The aim of study was to apply solvent/non-solvent treatment to coat the surface of 3D-printed bioresorbable poly(ε-caprolactone) scaffolds with bioactive hydroxyapatite particles and report on the physicochemical properties of the resulting materials.Material and Methods In the present study, biomimetic poly(ε-caprolactone) scaffolds were 3D-printed via fused deposition modeling technology and their surface was treated with the solvent/non-solvent method for coating with bioactive particles of hydroxyapatite.Results It has been found that treatment in the mixture of toluene and ethanol is suitable for the coating of poly(ε-caprolactone) scaffolds with hydroxyapatite. The scaffolds maintain porous structure after treatment while hydroxyapatite particles form homogeneous coating. The amount of hydroxyapatite on the treated scaffolds was 5.7 ± 0.8 wt. %.Discussion The proposed method ensures a homogeneous coating of outer and inner surfaces of the poly(ε-caprolactone) scaffolds with hydroxyapatite without a significant impact on the structure of a scaffold. Fourier-transform infrared spectroscopy confirmed that the solvent/non-solvent treatment has no effect on the chemical structure of PCL scaffolds.Conclusion Coating of biomimetic 3D-printed PCL scaffolds with bioactive hydroxyapatite by the solvent/non-solvent treatment has been successfully carried out. Upon coating, scaffolds retained their shape and interconnected porous structure and adsorbed hydroxyapatite particles that were uniformly distributed on the surface of the scaffold.
Bone tissue engineering, scaffolds, polycaprolactone, hydroxyapatite
Короткий адрес: https://sciup.org/142240027
IDR: 142240027 | DOI: 10.18019/1028-4427-2023-29-6-585-590
Список литературы Solvent/non-solvent treatment as a method for surface coating of poly(-caprolactone) 3D-printed scaffolds with hydroxyapatite
- Wubneh A, Tsekoura EK, Ayranci C, Uludag H. Current state of fabrication technologies and materials for bone tissue engineering. Acta Biomater. 2018;80:1-30. doi: 10.1016/j.actbio.2018.09.031
- Bharadwaz A, Jayasuriya AC. Recent trends in the application of widely used natural and synthetic polymer nanocomposites in bone tissue regeneration. Mater Sci Eng C Mater Biol Appl. 2020;110:110698. doi: 10.1016/j.msec.2020.110698
- Cheah CW, Al-Namnam NM, Lau MN, et al. Synthetic Material for Bone, Periodontal, and Dental Tissue Regeneration: Where Are We Now, and Where Are We Heading Next? Materials (Basel). 2021;14(20):6123. doi: 10.3390/ma14206123
- Yang X, Wang Y, Zhou Y, et al. The Application of Polycaprolactone in Three-Dimensional Printing Scaffolds for Bone Tissue Engineering. Polymers (Basel). 2021;13(16):2754. doi: 10.3390/polym13162754
- Gil-Castell O, Badia JD, Ontoria-Oviedo I, et al. In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case. Polymer Testing. 2018;66:256-267. doi: 10.1016/j.polymertesting.2018.01.027
- Fu Z, Cui J, Zhao B, et al. An overview of polyester/hydroxyapatite composites for bone tissue repairing. J Orthop Translat. 2021;28:118-130. doi: 10.1016/j.jot.2021.02.005
- Li Y, Liao C, Tjong SC. Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications. Nanomaterials (Basel). 2019 A;9(4):590. doi: 10.3390/nano9040590
- Gritsch L., Perrin E., Chenal J.M., et al. Combining bioresorbable polyesters and bioactive glasses: Orthopedic applications of composite implants and bone tissue engineering scaffolds. Appl Mater Today. 2021;22(13):100923. doi: 10.1016/j.apmt.2020.100923
- Gong L, Li J, Zhang J, et al. An interleukin-4-loaded bi-layer 3D printed scaffold promotes osteochondral regeneration. Acta Biomater. 2020;117:246-260. doi: 10.1016/j.actbio.2020.09.039
- Tian L, Zhang Z, Tian B, et al. Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds. RSC Adv. 2020;10(8):4805-4816. doi: 10.1039/c9ra10275b
- Cho YS, Quan M, Lee SH, et al. Assessment of osteogenesis for 3D-printed polycaprolactone/hydroxyapatite composite scaffold with enhanced exposure of hydroxyapatite using rat calvarial defect model. Compos Sci Technol. 2019;184:107844. doi: 10.1016/j.compscitech.2019.107844
- Bittner SM, Smith BT, Diaz-Gomez L, et al. Fabrication and mechanical characterization of 3D printed vertical uniform and gradient scaffolds for bone and osteochondral tissue engineering. Acta Biomater. 2019;90:37-48. doi: 10.1016/j.actbio.2019.03.041
- Gerdes S, Mostafavi A, Ramesh S, et al. Process-Structure-Quality Relationships of Three-Dimensional Printed Poly(Caprolactone)-Hydroxyapatite Scaffolds. Tissue Eng Part A. 2020;26(5-6):279-291. doi: 10.1089/ten.TEA.2019.0237
- Eosoly S, Vrana NE, Lohfeld S, et al. Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS). Mater Sci Eng: C. 2012;32(8):2250-2257. doi: 10.1016/j.msec.2012.06.011
- Suo H, Chen Y, Liu J, et al. 3D printing of biphasic osteochondral scaffold with sintered hydroxyapatite and polycaprolactone. J Mater Sci. 2021;56:16623-16633. doi: 10.1007/s10853-021-06229-x
- Cho YS, Quan M, Kang NU, et al. Strategy for enhancing mechanical properties and bone regeneration of 3D polycaprolactone kagome scaffold: Nano hydroxyapatite composite and its exposure. Eur Polym J. 2020;134:109814. https://doi.org/10.1016/j.eurpolymj.2020.109814.
- Gomez-Lizarraga KK, Flores-Morales C, Del Prado-Audelo ML, et al. Polycaprolactone- and polycaprolactone/ceramic-based 3D-bioplotted porous scaffolds for bone regeneration: A comparative study. Mater Sci Eng C Mater Biol Appl. 2017;79:326-335. doi: 10.1016/j.msec.2017.05.003
- Liao HT, Lee MY, Tsai WW, et al. Osteogenesis of adipose-derived stem cells on polycaprolactone-ß-tricalcium phosphate scaffold fabricated via selective laser sintering and surface coating with collagen type I. J Tissue Eng Regen Med. 2016;10(10):E337-E353. doi: 10.1002/term.1811
- Arif ZU, Khalid MY, Noroozi R, et al. Recent advances in 3D-printed polylactide and polycaprolactone-based biomaterials for tissue engineering applications. Int J Biol Macromol. 2022;218:930-968. doi: 10.1016/j.ijbiomac.2022.07.140
- Jaroszewicz J, Idaszek J, Choinska E, et al. Formation of calcium phosphate coatings within polycaprolactone scaffolds by simple, alkaline phosphatase based method. Mater Sci Eng C Mater Biol Appl. 2019;96:319-328. doi: 10.1016/j.msec.2018.11.027
- Goreninskii SI, Stankevich KS, Nemoykina AL, et al. A first method for preparation of biodegradable fibrous scaffolds containing iodine on the fibre surfaces. Bull Mater Sci. 2018;41:100. doi: 10.1007/s12034-018-1625-z
- Elzein T, Nasser-Eddine M, Delaite C, et al. FTIR study of polycaprolactone chain organization at interfaces. J Colloid Interface Sci. 2004;273(2):381-7. doi: 10.1016/j.jcis.2004.02.001
- Sabino MA. Oxidation of polycaprolactone to induce compatibility with other degradable polyesters. Polym Degrad Stab. 2007;92(6):986-996. doi: 10.1016/j.polymdegradstab.2007.03.010
- Dias JR, Sousa A, Augusto A, et al. Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study. Polymers (Basel). 2022;14(16):3397. doi: 10.3390/polym14163397
- Woodard LN, Grunlan MA. Hydrolytic Degradation and Erosion of Polyester Biomaterials. ACS Macro Lett. 2018;7(8):976-982. doi: 10.1021/ acsmacrolett.8b00424
- Tobita H. Random degradation of branched polymers. 2. Multiple branches. Macromolecules. 1996;29:3010-3021. doi: 10.1021/ma9509725.
- Tang Z, Li X, Tan Y, et al. The material and biological characteristics of osteoinductive calcium phosphate ceramics. Regen Biomater. 2018;5(1):43-59. doi: 10.1093/rb/rbx024
- Dorozhkin SV. Functionalized calcium orthophosphates (CaPO4) and their biomedical applications. J Mater Chem B. 2019;7(47):7471-7489. doi: 10.1039/c9tb01976f
- Koju N, Sikder P, Ren Y, et al. Biomimetic coating technology for orthopedic implants. Curr Opin Chem Eng. 2017;15:49-55. doi: 10.1016/j. coche.2016.11.005
- Arcos D, Vallet-Regi M. Substituted hydroxyapatite coatings of bone implants. J Mater Chem B. 2020;8(9):1781-1800. doi: 10.1039/c9tb02710f
- Rezaei A, Mohammadi MR. In vitro study of hydroxyapatite/polycaprolactone (HA/PCL) nanocomposite synthesized by an in situ sol-gel process. Mater Sci Eng C Mater Biol Appl. 2013;33(1):390-396. doi: 10.1016/j.msec.2012.09.004
- Schneider M, Fritzsche N, Puciul-Malinowska A, et al. Surface Etching of 3D Printed Poly(lactic acid) with NaOH: A Systematic Approach. Polymers (Basel). 2020;12(8):1711. doi: 10.3390/polym12081711
