Compatible solutes of the strain Halomonas sp. SMB31, synthesized in response to osmotic stress

Study of the physiology of the strain Halomonas sp. SMB31 showed that it grew in Raymond mineral medium with glucose (1 g / l) as a substrate in a wide range of sodium chloride concentrations of 1-20%, with a growth optimum at 5% NaCl. Based on the data obtained, the strain was assigned to the group of moderately halophilic bacteria. Examination of cell ethanol extracts of the strain Halomonas sp. SMB31 grown in the presence of elevated salt concentrations (5, 10, 15% NaCl) by NMR 1H spectroscopy showed that the predominant compatible solute was ectoine. In addition, glutamic acid was present in the cells. An increase in the salinity of the culture medium from 5% to 10% NaCl led to the accumulation of hydroxyectoine by the cells. It was found that the intracellular amount of ectoine of the strain Halomonas sp. SMB31 depended on the concentration of sodium chloride and the growth phase of the bacterial culture.