Современные методы исследования апоптоза с применением проточной цитофлюориметрии

Автор: Власова Виолетта Викторовна, Сайдакова Евгения Владимировна

Журнал: Вестник Пермского университета. Серия: Биология @vestnik-psu-bio

Статья в выпуске: 4, 2018 года.

Бесплатный доступ

В процессе апоптоза - программной гибели - клетка претерпевает ряд консервативных биохимических изменений. Среди них ключевыми событиями являются потеря трансмембранного потенциала митохондрий, активация проапоптотических белков семейства Bcl-2, нарушение липидной асимметрии и повышение проницаемости цитоплазматической мембраны, активация ферментов из семейства каспаз и фрагментация нуклеиновых кислот. Многоцветная проточная цитофлюоримет-рия позволяет детектировать большинство из событий, свидетельствующих об индукции программной гибели клетки, что дает возможность исследования апоптоза на самых ранних его этапах. В настоящей статье описаны основные подходы к детекции апоптоза с применением многоцветной проточной цитофлюориметрии; рассмотрены молекулярные процессы, лежащие в их основе; обозначены преимущества и ограничения в использовании различных методов определения программной гибели клеток в биологии и медицине.

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Проточная цитофлюориметрия, апоптоз, митохондрии, проницаемость цитоплазматической мембраны, асимметрия цитоплазматической мембраны, каспазы, фрагментация днк

Короткий адрес: https://sciup.org/147227052

IDR: 147227052 | DOI: 10.17072/1994-9952-2018-4-430-442

Список литературы Современные методы исследования апоптоза с применением проточной цитофлюориметрии

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