The development of turnip mosaic virus resistant doubled haploid lines of Brassica napus L

Oilseed rape is a valuable crop used for biodiesel, vegetable oil and high-protein animal feed. However, there is a need for cultivars resistant to turnip mosaic virus (TuMV), which causes a great losses. Resistant plants are the only efficient and environmentally safe way to control this virus. We have studied the resistance in collection of 64 accessions of cultivated and wild brassicas ( Brassica napus, B. rapa, B. oleracea and B. juncea to six genetically different isolates of TuMV. Assessment of visual mosaic symptoms and quantitative ELISA test identified 24 most promising accessions that were resistant to all isolates of TuMV (13 accessions) and 5 of 6 isolates (11 accessions). Then, the haploid resistant plants were obtained using microspore culture. An optimal condition of high temperature treatment to induce embryogenesis were found for 12 accessions, and doubled haploid lines, the donors of resistance to TuMV, were propagated.