Establishment of an orthotopic model of endometrial cancer

Автор: Frantsiyants E.M., Shikhlyarova A.I., Kaplieva I.V., Bandovkina V.A., Pogorelova Yu.A., Neskubina I.V., Trepitaki L.K., Surikova E.I., Moiseenko T.I., Cheryarina N.D., Kotieva V.M., Vereskunova A.A., Menshenina A.P., Rogozin M.A., Ishonina O.G., Ushakova N.D.

Журнал: Сибирский онкологический журнал @siboncoj

Рубрика: Лабораторные и экспериментальные исследования

Статья в выпуске: 6 т.23, 2024 года.

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The purpose of this study was to establish a model of uterine carcinoma in female laboratory rats by transplanting Guerin carcinoma directly into the uterine horn. Material and Methods. Fifteen nonlinear white laboratory rats weighing 250 ± 25 g served as the subjects of surgical intervention. All operative interventions were performed under xylazine-zoletil anesthesia. Female white laboratory rats were laparotomized under aseptic conditions using anesthesia. The incision length was 2 cm, and a tumor suspension containing 2.5-3.5×106 cells was injected into the lumen of the right uterine horn using an intravenous catheter with a 22G injection port (0.9 × 25 mm). Tumor cells were counted using the ADAMIILS cell analyzer (Nano Entek, Korea). The tumor progression was monitored for 21 days. After euthanizing the animals under ether anesthesia, median longitudinal histological sections, 5-7 μm thick, were made from the tumor node and stained with hematoxylin-eosin and Van-Gizon using standard techniques.

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Experimental model, endometrial cancer, malignant growth, guerin carcinoma, tumor node, female rats, morphological study

Короткий адрес: https://sciup.org/140308740

IDR: 140308740   |   DOI: 10.21294/1814-4861-2024-23-6-70-80

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