Spectrophotometric control of connection optically transparent materials and thin layers

One of the important tasks while creating biosensor and microfluidic devices is to control the quality of sensitive layer fixation needed to achieve the required functional characteristics. Among the methods of non-destructive testing of the surface properties of materials are favorably allocated optical spectrophotometry as simple, reliable and the most accessible for the research laboratory. We consider a spectrophotometric method for control of coupling of film micron sizes with an optically transparent substrate. The proposed approach is demonstrated by the example of control fixation polyacrylamide gel on the surface of a plate of polymethylmethacrylate, which has previously been subjected to chemical (or physical) treatment and silanization.