Method for modeling a skin wound in pigs in experiment

Aim: development of an experimental model for studying the course of the wound process and the effectiveness of applying wound dressings both in clean conditions and on a purulent wound in one animal. Material and Methods. Pig wound modeling is used as an in vivo model. In the operating room, under general anesthesia in the lateral position, the laboratory animal is marked with the future burn surface. A hot steel contact pad applies a thermal injury to the skin of a given size and the required depth of thermal injury (IIIB and IV grades according to Vishnevsky’s classification). Then necrectomy was performed with primary and delayed plasty using the studied wound dressings. After this, non-viable tissues are removed with primary and delayed autodermoplasty and plastic wound dressings. The research subject — in vivo study included 4 Landrace pigs (animal weight from 18 to 24 Kg). Results. With burns IIIB Art. under the decellularized scaffolds, scar tissue was formed by the 12th day, under the recellularized ones by the 8th day, epithelialization of the wound occurred. With burns IV Art. granulation tissue without aseptic inflammation on the 8th day was formed. Conclusion. The developed method for modeling a wound in an experiment allows one animal to analyze the engraftment of wound coverings, both in early and in staged surgical treatment of burns.