The comparative analysis of two alternative chromatographic purification methods for manufacture of preparation of human coagulation factor VIII at a dosage of 500 international units / vial
Автор: Yamkin A.V., Stronin O.V., Nikitina L.N., Semenova N.A., Epanchintsev A.A., Shkvyrya N.S.
Журнал: Сибирский журнал клинической и экспериментальной медицины @cardiotomsk
Рубрика: Лабораторные и экспериментальные исследования
Статья в выпуске: 2-2 т.26, 2011 года.
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Two methods of chromatographic purification for manufacture of preparation of human coagulation factor VIII at a dosage of 500 IU/vial were compared. Size exclusion chromatography and ion-exchange chromatography were used in method 1, ion-exchange chromatography only was used in method 2. It was found that despite lower recovery, method 1 provided higher level of purification of factor VIII in comparison with method 2. Thus, purification method 1 is preferable for preparation of human coagulation factor VIII.
Фактор свертывания viii, coagulation factor viii, size exclusion chromatography, ion exchange chromatography
Короткий адрес: https://sciup.org/14919482
IDR: 14919482
