Glass-polymeric microfluidic chips for electrophoretic separation of biomolecules

The results of creation of the prototype glass-polymeric microfluidic chip for rapid analysis of biomolecules by electrophoresis are discussed. As the main material was used the polydimethylsiloxane Sylgard 184, in which was formed a network of microchannels by soft lithography. Are discussed the results obtained after different methods of physico-chemical treatment of the working surfaces of the channels of the microfluidic chips and the estimated effect of processing methods on the electroosmotic flow. Microfluidic chips was tested at the electrophoretic separation of a mixture of oligonucleotides 10, 20, 30, 40 and 50 bases. Based on the electrophoregrams analysis a model of information signals (peaks) and noise was proposed. The basic theoretical principles that define the mechanism of convective-diffusive transport of the mixture components were checked.