Experimental stereological analysis of the tibial anterior muscle and endomysium capillaries with graduated leg lengthening by four-fold and automatic division

Comparative stereological analysis of the anterior tibial muscle and endomysium capillaries of experimental and contralateral limbs with different divisions of the leg distraction used was made in adult mongrel dogs experimentally. The clinical picture for autodistraction was shown to be much better in comparison with 4-fold division due to better blood supply of the muscular tissue for high-division lengthening. In case of 4-fold division both the less numerical density of microvessels and the parameter, reflecting the muscle vascularization, are compensated by the greater area of hematotissular diffusion. The ultrastructure of endothelial capillaries also affirms different adaptation processes, associated with the conditions of leg distraction. Great extent of the zones of the intercellular contacts of endotheliocytes in the area of endothelial mark during distraction of 4-fold division can show the increase of endotheocyte adhesive properties, and the shares of areas, occupied by the marks of the cisternae of granular endoplasmic reticulum and mitrochondria in the area of endothelial mark define high metabolic activity and intensification of endotheliocyte oxidation-reduction processes comparing to autodistraction.