Study of dendritic cell migration using CELL-IQ analysis system
Автор: Nekhaeva Tatyana L., Danilova Anna B., Baldueva Irina A.
Журнал: Сибирский онкологический журнал @siboncoj
Рубрика: Лабораторные и экспериментальные исследования
Статья в выпуске: 4 т.17, 2018 года.
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Dendritic cells (DCs) belong to specialized pool of antigen-presenting cells with high functional plasticity and manifest with immunostimulatory or immunosuppressive potential depending on sequence and combination of microenvironment stimuli, which determine their differentiation, maturation and activation. The use of antitumor DCs vaccines is based on the ability of DCs specifically activated in vitro migrate for antigen presentation to T-lymphocytes. We studied the components of the tumor microenvironment that are capable of inhibiting DCs migration. The study of the mobility of DCs in Cell-IQ experimental analytical system showed the presence of an inverse correlation of high strength between the average trajectory speed and the level of immunosuppressive factors (ISFs) in supernatants of cultured skin melanoma cells (TgFβ1, IL-10, IL-18, VEgF-A, EgF, FgF, HgF, sFASL (p
Dendritic cells, migration, immunosuppressive factors, cancer testis antigens
Короткий адрес: https://sciup.org/140254193
IDR: 140254193 | DOI: 10.21294/1814-4861-2018-17-4-14-23
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